Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on December 15, 2006; 10.1104/pp.106.089615

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
143/2/1044    most recent
pp.106.089615v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jablonska, B.
Right arrow Articles by Kaloshian, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jablonska, B.
Right arrow Articles by Kaloshian, I.
Agricola
Right arrow Articles by Jablonska, B.
Right arrow Articles by Kaloshian, I.

Received September 7, 2006
Accepted December 8, 2006

The Mi-9 Gene from Solanum arcanum Conferring Heat-Stable Resistance to Root-Knot Nematodes Is a Homologue of Mi-1

Barbara Jablonska , Jetty S. S. Ammiraju , Kishor K. Bhattarai , Sophie Mantelin , Oscar Martinez de Ilarduya , Philip A. Roberts , and Isgouhi Kaloshian

Department of Nematology, University of California, Riverside, CA 92521

Resistance conferred by the Mi-1 gene from Solanum peruvianum is effective and widely used for limiting root-knot nematode (Meloidogyne spp.) yield loss in tomato (Solanum lycopersicum), but the resistance is ineffective at soil temperatures above 28°C. Previously, we mapped the heat-stable resistance gene Mi-9 in Solanum arcanum accession LA2157 to the short arm of chromosome 6, in a genetic interval as Mi-1 and the Cladosporium fulvum resistance gene Cf2. We developed a fine map of the Mi-9 region by resistance and marker screening of an F2 population and derived F3 families from resistant LA2157 x susceptible LA392. Mi-1 intron 1 flanking primers were designed to amplify intron 1 and fingerprint Mi-1 homologues. Using these primers, we identified seven Mi-1 homologues in the mapping parents. Cf-2 and Mi-1 homologues were mapped on chromosome 6 using a subset of the F2. Cf-2 homologues did not segregate with Mi-9 resistance, but three Mi-1 homologues (RH1, RH2, and RH4) from LA2157 and one (SH1) from LA392 co-localized to the Mi-9 region. Reverse transcriptase PCR analysis indicated that six Mi-1 homologues are expressed in LA2157 roots. We targeted transcripts of Mi-1 homologues for degradation with tobacco rattle virus (TRV)-based virus-induced gene silencing using Agrobacterium infiltration with a TRV-Mi construct. In most LA2157 plants infiltrated with the TRV-Mi construct, Mi-9 meditated heat-stable root-knot nematode resistance was compromised at 32°C indicating that the heat-stable resistance is mediated by a homologue of Mi-1.




This article has been cited by other articles:


Home page
 J Exp BotHome page
G. Dubreuil, M. Magliano, M. P. Dubrana, J. Lozano, P. Lecomte, B. Favery, P. Abad, and M. N. Rosso
Tobacco rattle virus mediates gene silencing in a plant parasitic root-knot nematode
J. Exp. Bot., October 1, 2009; 60(14): 4041 - 4050.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists