Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 QUICK SEARCH:   [advanced]


     


Plant Physiology Preview
Published on November 3, 2006; 10.1104/pp.106.090050


OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
143/1/364    most recent
pp.106.090050v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (30)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tian, M.
Right arrow Articles by Kamoun, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tian, M.
Right arrow Articles by Kamoun, S.
Agricola
Right arrow Articles by Tian, M.
Right arrow Articles by Kamoun, S.

Received September 19, 2006
Accepted October 24, 2006

A Phytophthora Infestans Cystatin-like Protein Targets a Novel Tomato Papain-like Apoplastic Protease

Miaoying Tian , Joe Win , Jing Song , Renier van der Hoorn , Esther van der Knaap , and Sophien Kamoun *

Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA
Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany
Department of Horticulture and Crop Sciences, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA

* Corresponding author; email: kamoun.1{at}osu.edu.

There is emerging evidence that the proteolytic machinery of plants plays important roles in defense against pathogens. The oomycete pathogen Phytophthora infestans, the agent of the devastating late blight disease of tomato and potato, has evolved an arsenal of protease inhibitors to overcome the action of host proteases. Previously, we described a family of fourteen Kazal-like extracellular serine protease inhibitors from P. infestans. Among these, EPI1 and EPI10 bind and inhibit the pathogenesis-related (PR) P69B subtilisin-like serine protease of tomato. Here, we describe EPIC1-EPIC4, a new family of P. infestans secreted proteins with similarity to cystatin-like protease inhibitor domains. Among these, the epiC1 and epiC2 genes lacked orthologs in Phytophthora sojae and Phytophthora ramorum, were relatively fast-evolving within P. infestans, and were upregulated during infection of tomato, suggesting a role during P. infestans-host interactions. Biochemical functional analyses revealed that EPIC2B interacts with and inhibits a novel papain-like extracellular cysteine protease, termed Phytophthora Inhibited Protease 1 (PIP1). Characterization of PIP1 revealed that it is a PR protein closely related to Rcr3, a tomato apoplastic cysteine protease that functions in fungal resistance. Altogether, this and earlier studies suggest that interplay between host proteases of diverse catalytic families and pathogen inhibitors is a general defense-counterdefense process in plant-pathogen interactions.




This article has been cited by other articles:


Home page
Plant Physiol.Home page
L. W. Neuteboom, K. O. Matsumoto, and D. A. Christopher
An Extended AE-Rich N-Terminal Trunk in Secreted Pineapple Cystatin Enhances Inhibition of Fruit Bromelain and Is Posttranslationally Removed during Ripening
Plant Physiology, October 1, 2009; 151(2): 515 - 527.
[Abstract] [Full Text] [PDF]


Home page
Plant CellHome page
S.-K. Oh, C. Young, M. Lee, R. Oliva, T. O. Bozkurt, L. M. Cano, J. Win, J. I.B. Bos, H.-Y. Liu, M. van Damme, et al.
In Planta Expression Screens of Phytophthora infestans RXLR Effectors Reveal Diverse Phenotypes, Including Activation of the Solanum bulbocastanum Disease Resistance Protein Rpi-blb2
PLANT CELL, September 1, 2009; 21(9): 2928 - 2947.
[Abstract] [Full Text] [PDF]


Home page
Mol Biol EvolHome page
A. Zamora, Q. Sun, M. T. Hamblin, C. F. Aquadro, and S. Kresovich
Positively Selected Disease Response Orthologous Gene Sets in the Cereals Identified Using Sorghum bicolor L. Moench Expression Profiles and Comparative Genomics
Mol. Biol. Evol., September 1, 2009; 26(9): 2015 - 2030.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
J. Song, J. Win, M. Tian, S. Schornack, F. Kaschani, M. Ilyas, R. A. L. van der Hoorn, and S. Kamoun
Apoplastic effectors secreted by two unrelated eukaryotic plant pathogens target the tomato defense protease Rcr3
PNAS, February 3, 2009; 106(5): 1654 - 1659.
[Abstract] [Full Text] [PDF]


Home page
Plant CellHome page
R. A.L. van der Hoorn and S. Kamoun
From Guard to Decoy: A New Model for Perception of Plant Pathogen Effectors
PLANT CELL, August 1, 2008; 20(8): 2009 - 2017.
[Abstract] [Full Text] [PDF]


Home page
Plant CellHome page
H. P. van Esse, J. W. van't Klooster, M. D. Bolton, K. A. Yadeta, P. van Baarlen, S. Boeren, J. Vervoort, P. J.G.M. de Wit, and B. P.H.J. Thomma
The Cladosporium fulvum Virulence Protein Avr2 Inhibits Host Proteases Required for Basal Defense
PLANT CELL, July 1, 2008; 20(7): 1948 - 1963.
[Abstract] [Full Text] [PDF]


Home page
Plant CellHome page
M. Shabab, T. Shindo, C. Gu, F. Kaschani, T. Pansuriya, R. Chintha, A. Harzen, T. Colby, S. Kamoun, and R. A.L. van der Hoorn
Fungal Effector Protein AVR2 Targets Diversifying Defense-Related Cys Proteases of Tomato
PLANT CELL, April 1, 2008; 20(4): 1169 - 1183.
[Abstract] [Full Text] [PDF]


Home page
Plant Physiol.Home page
J. A. Lindbo
TRBO: A High-Efficiency Tobacco Mosaic Virus RNA-Based Overexpression Vector
Plant Physiology, December 1, 2007; 145(4): 1232 - 1240.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists