Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on April 13, 2007; 10.1104/pp.106.090241

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
144/2/1079    most recent
pp.106.090241v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schaff, J. E.
Right arrow Articles by Bird, D. Mck.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schaff, J. E.
Right arrow Articles by Bird, D. Mck.
Agricola
Right arrow Articles by Schaff, J. E.
Right arrow Articles by Bird, D. Mck.

Received September 22, 2006
Accepted March 31, 2007

Comprehensive Transcriptome Profiling in Tomato Reveals a Role for Glycosyltransferase in Mi-mediated Nematode Resistance

Jennifer E. Schaff , Dahlia M. Nielsen , Chris P. Smith , Elizabeth H. Scholl , and David Mck. Bird *

NC State University, Department of Plant Pathology; NC State University, Department of Genetics; NC State University, Bioinformatics Research Center

* Corresponding author; email: david_bird{at}ncsu.edu.

Root-knot nematode (RKN: Meloidogyne spp.) is a major crop pathogen worldwide. Effective resistance exists for a few plant species, including that conditioned by Mi in tomato (Solanum lycopersicum). We interrogated the root transcriptome of the resistant (Mi+) and susceptible (Mi-) cultivars Motelle and Moneymaker respectively during a time-course infection by the Mi-susceptible RKN species, M. incognita, and the Mi-resistant species, M. hapla. In the absence of RKN infection, only a single significantly regulated gene, encoding a glycosyltransferase, was detected. However, RKN infection influenced the expression of broad suites of genes; more than half of the probes on the array identified differential gene regulation between infected and uninfected root tissue at some stage of RKN infection. We discovered 217 genes regulated during the time of RKN infection corresponding to establishment of feeding sites, and 58 genes that exhibited differential regulation in resistant roots compared to uninfected roots, including the glycosyltransferase. Using VIGS to silence the expression of this gene restored susceptibility to Meloidogyne incognita in Motelle, indicating that this gene is necessary for resistance to RKN. Collectively, our data provide a picture of global gene expression changes in roots during compatible and incompatible associations with RKN, and point to candidates for further investigation.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists