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Published on December 1, 2006; 10.1104/pp.106.090712

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Received October 2, 2006
Accepted November 26, 2006

Heat Stress Induces an Aggregation of the Light-harvesting Complex of Photosystem II in Spinach Plants

Yunlai Tang , Xiaogang Wen , Qingtao Lu , Zhipan Yang , Zhukuan Cheng , and Congming Lu *

Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China

* Corresponding author; email: lucm{at}ibcas.ac.cn.

Whole spinach plants were subjected to heat stress (25-50°C) in the dark for 30 min. At temperatures higher than 35°C, CO2 assimilation rate decreased significantly. The maximal efficiency of PSII photochemistry remained unchanged until 45°C and decreased only slightly at 50°C. Non-photochemical quenching increased significantly either in the absence or presence of dithiothreitol. There was an appearance of the characteristic band at around 698 nm in 77K fluorescence emission spectra of leaves. Native green-gel of thylakoid membranes isolated immediately from heat-stressed leaves showed that much pigment-protein complexes remained aggregated in the stacking gel. The analyses of SDS-PAGE and immunoblotting demonstrated that the aggregates were composed of the main light-harvesting complex of PSII (LHCIIb). To characterize the aggregates, isolated PSII core complexes were incubated at 25-50°C in the dark for 10 min. At temperatures over 35°C, much pigment-protein complexes remained aggregated in the stacking gel of native green-gel and immunoblotting analyses showed that the aggregates were composed of LHCIIb. In addition, isolated LHCII was also incubated at 25-50°C in the dark for 10 min. Much LHCII remained aggregated in the stacking gel of native green-gel at temperatures over 35°. Massive aggregation of LHCII was clearly observed by using microscope images, which was companied by a significant increase in fluorescence quenching. There was a linear relationship between the formation of LHCII aggregates and non-photochemical quenching in vivo. The results in this study suggest that LHCII aggregates may represent a protective mechanism to dissipate excess excitation energy in heat-stressed plants.




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