Plant Physiology Preview
Published on January 12, 2007; 10.1104/pp.106.091298
OPEN ACCESS ARTICLE
Received October 12, 2006
Accepted December 28, 2006
The coi1 Mutation Reveals the Hormonal Signaling Interaction Between ABA and MeJA in Arabidopsis Guard Cells. Specific Impairment of Ion Channel Activation and Second Messenger Production
Shintaro Munemasa , Kenji Oda , Megumi Watanabe-Sugimoto , Yoshimasa Nakamura , Yasuaki Shimoishi , and Yoshiyuki Murata *
The Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Okayama 700-8530 Japan; Research Institute for Biological Sciences OKAYAMA, Kibichuo-cho, Okayama 716-1241 Japan; Department of Agriculture, Okayama University, Tsushima-Naka, Okayama 700-8530 Japan
* Corresponding author; email: muta{at}cc.okayama-u.ac.jp.
Methyl jasmonate (MeJA) elicits stomatal closing similar to abscisic acid (ABA) but whether the two compounds use similar or different signaling mechanisms in guard cells remains to be clarified. We investigated the effects of MeJA and ABA on second messenger production and ion channel activation in guard cells of wild-type Arabidopsis and MeJA-insensitive coi1 mutants. The coi1 mutation impaired MeJA-induced stomatal closing but not ABA-induced stomatal closing. MeJA as well as ABA induced production of reactive oxygen species (ROS) and nitric oxide (NO) in wild-type guard cells whereas MeJA did not induce production of ROS and NO in coi1 guard cells. The experiments using an inhibitor and scavengers demonstrated that both ROS and NO are involved in MeJA-induced stomatal closing as well as ABA-induced stomatal closing. Not only ABA but also MeJA activated slow (S-type) anion channels and Ca2+ permeable cation (ICa) channels in the plasma membrane of wild-type guard cell protoplasts (GCPs). However, in coi1 GCPs, MeJA did not elicit either S-type anion currents or ICa currents but ABA activated both types of ion channels. Furthermore in order to elucidate signaling interaction between ABA and MeJA in guard cells, we examined MeJA signaling in ABA-insensitive mutant abi2-1 whose ABA signal transduction cascade has some disruption downstream of ROS production and NO production. MeJA also did not induce stomatal closing but stimulated production of ROS and NO in abi2-1. These results suggest that MeJA triggers stomatal closing via a receptor distinct from the ABA receptor and that the coi1 mutation disrupts MeJA signaling upstream of the blanch point of ABA signaling and MeJA signaling in Arabidopsis guard cells.
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