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Published on December 22, 2006; 10.1104/pp.106.091488

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Received October 19, 2006
Accepted November 22, 2006

MPB2C, a Microtubules-associated Plant Factor, Is Required for Microtubular Accumulation of Tobacco Mosaic Virus Movement Protein in Plants

Mirela Curin , Eve-Ly Ojangu , Kateryna Trutnyeva , Birger Ilau , Erkki Truve , and Elisabeth Waigmann *

Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Medical Biochemistry, Medical University of Vienna, Dr. Bohrgasse 9, A-1030 Vienna, Austria; Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086 Tallinn, Estonia

* Corresponding author; email: elisabeth.waigmann{at}meduniwien.ac.at.

MPB2C is a plant endogenous microtubule associated protein previously identified as an interaction partner of tobacco mosaic virus movement protein (TMV-MP). In this work, the role of MPB2C in cell-to-cell transport of TMV-MP, viral spread of TMV and subcellular localization of TMV-MP was examined. To this end, plants with reduced MPB2C levels were generated by a gene silencing strategy. Local and systemic spread of TMV and cell-to-cell movement of TMV-MP were unimpaired in MPB2C silenced plants as compared to non-silenced plants, indicating that MPB2C is not required for intercellular transport of TMV-MP itself or spread of tobacco mosaic virus. However, a clear change in subcellular distribution of TMV-MP characterized by a nearly complete loss of microtubular localization was observed in MPB2C silenced plants. This result shows that the MPB2C is a central player in determining the complex subcellular localization of TMV-MP, in particular its microtubular accumulation, a phenomenon that has been frequently observed and whose role is still under discussion Clearly, MPB2C mediated accumulation of TMV-MP at microtubules is not required for intercellular spread, but may be a means to withdraw the TMV-MP from the cell-to-cell transport pathway.




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