Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on November 17, 2006; 10.1104/pp.106.091496

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
143/1/461    most recent
pp.106.091496v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Laloi, M.
Right arrow Articles by Moreau, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laloi, M.
Right arrow Articles by Moreau, P.
Agricola
Right arrow Articles by Laloi, M.
Right arrow Articles by Moreau, P.

Received October 18, 2006
Accepted November 10, 2006

Insights into the Role of Specific Lipids in the Formation and Delivery of Lipid Microdomains to the Plasma Membrane of Plant Cells

Maryse Laloi , Anne-Marie Perret , Laurent Chatre , Su Melser , Catherine Cantrel , Marie-Noëlle Vaultier , Alain Zachowski , Katell Bathany , Jean-Marie Schmitter , Myriam Vallet , René Lessire , Marie-Andrée Hartmann , and Patrick Moreau *

Laboratoire de Biogenèse Membranaire, UMR 5200-CNRS-Université Victor Segalen Bordeaux 2, 146, rue Léo Saignat, BP 33076 Bordeaux Cedex, France
Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes, FRE CNRS 2846, Université Pierre et Marie Curie, site "le Raphaël", 3 rue Galilée, 94200 Ivry-sur-Seine, France
Institut Européen de Chimie et Biologie, 2 rue Robert Escarpit, 33600 Pessac, France
Institut de Biologie Moléculaire des Plantes, UPR-CNRS 2357, 67084 Strasbourg Cedex, France

* Corresponding author; email: pmoreau{at}biomemb.u-bordeaux2.fr.

The existence of sphingolipid- and sterol-enriched microdomains, known as lipid rafts, in the plasma membrane of eukaryotic cells is well documented. In order to obtain more insights into the lipid molecular species required for the formation of microdomains in plants, we have isolated detergent(Triton X-100)-resistant membranes (DRMs) from the plasma membrane (PM) of Arabidopsis thaliana and Allium porrum seedlings as well as from Arabidopsis thaliana cell cultures. Here we show that all DRM preparations are enriched in sterols, sterylglucosides and glucosylceramides, and depleted in glycerophospholipids. The glucosylceramides of DRMs from A. porrum seedlings contain hydroxypalmitic acid. We investigated the role of sterols in DRM formation along the secretory pathway in A. porrum seedlings. We present evidence for the presence of DRMs in both the PM and the Golgi apparatus, but not in the endoplasmic reticulum. In leek seedlings treated with fenpropimorph, a sterol biosynthesis inhibitor, the usual {Delta}5-sterols are replaced by 9{beta},19-cyclopropylsterols. In these plants, sterols and hydroxypalmitic acid-containing glucosylceramides do not reach the PM, and most DRMs are recovered from the Golgi apparatus, indicating that {Delta}5-sterols and glucosylceramides play a crucial role in lipid microdomain formation and delivery to the PM. In addition, DRM formation in A. thaliana cells is shown to depend on the unsaturation degree of fatty acyl chains as attested by the dramatic decrease in the amount of DRMs prepared from the Arabidopsis mutants, fad2 and Fad3+, affected in their fatty acid desaturases.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
M. Fujiwara, S. Hamada, M. Hiratsuka, Y. Fukao, T. Kawasaki, and K. Shimamoto
Proteome Analysis of Detergent-Resistant Membranes (DRMs) Associated with OsRac1-Mediated Innate Immunity in Rice
Plant Cell Physiol., July 1, 2009; 50(7): 1191 - 1200.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Raffaele, E. Bayer, D. Lafarge, S. Cluzet, S. German Retana, T. Boubekeur, N. Leborgne-Castel, J.-P. Carde, J. Lherminier, E. Noirot, et al.
Remorin, a Solanaceae Protein Resident in Membrane Rafts and Plasmodesmata, Impairs Potato virus X Movement
PLANT CELL, May 1, 2009; 21(5): 1541 - 1555.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Minami, M. Fujiwara, A. Furuto, Y. Fukao, T. Yamashita, M. Kamo, Y. Kawamura, and M. Uemura
Alterations in Detergent-Resistant Plasma Membrane Microdomains in Arabidopsis thaliana During Cold Acclimation
Plant Cell Physiol., February 1, 2009; 50(2): 341 - 359.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Zauner, U. Zahringer, B. Lindner, D. Warnecke, and P. Sperling
Identification and Functional Characterization of the 2-Hydroxy Fatty N-Acyl-{Delta}3(E)-desaturase from Fusarium graminearum
J. Biol. Chem., December 26, 2008; 283(52): 36734 - 36742.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Raffaele, S. Mongrand, P. Gamas, A. Niebel, and T. Ott
Genome-Wide Annotation of Remorins, a Plant-Specific Protein Family: Evolutionary and Functional Perspectives
Plant Physiology, November 1, 2007; 145(3): 593 - 600.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Lefebvre, F. Furt, M.-A. Hartmann, L. V. Michaelson, J.-P. Carde, F. Sargueil-Boiron, M. Rossignol, J. A. Napier, J. Cullimore, J.-J. Bessoule, et al.
Characterization of Lipid Rafts from Medicago truncatula Root Plasma Membranes: A Proteomic Study Reveals the Presence of a Raft-Associated Redox System
Plant Physiology, May 1, 2007; 144(1): 402 - 418.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists