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Published on January 19, 2007; 10.1104/pp.106.092148

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Received November 7, 2006
Accepted January 9, 2007

Developmental Analysis of Maize Endosperm Proteome Suggests a Pivotal Role for PPDK

Valérie Méchin *, Claudine Thévenot , Martine Le Guilloux , Jean-Louis Prioul , and Catherine Damerval

UMR206 Chimie Biologique, INRA - INA PG, F-78850 Thiverval Grignon; Institut de Biotechnologie des Plantes, Université Paris Sud, F-91405 Orsay; UMR de Génétique Végétale, INRA - Univ. Paris Sud - CNRS - INA PG, F-91190 Gif sur Yvette

* Corresponding author; email: mechin{at}grignon.inra.fr.

Although the morphological steps of maize endosperm development are well described, very little is known concerning the coordinated accumulation of the numerous proteins involved. Here, we present the first proteomic study of maize endosperm development. The accumulation pattern of 409 proteins at seven developmental stages was examined. Hierarchical clustering analysis allowed four main developmental profiles to be recognized. Comprehensive investigation of the functions associated to clusters resulted in a consistent picture of the developmental coordination of cellular processes. Early stages, devoted to cellularisation, cell division and cell wall deposition, corresponded to maximum expression of actin, tubulins and cell organisation proteins, of respiration metabolism (glycolysis and TCA cycle) and of protection against reactive oxygen species. An important protein turnover which is likely associated to the switch from growth and differentiation to storage was also suggested from the high amount of proteases. A relative increase of abundance of the glycolytic enzymes compared to TCA enzymes is consistent with the recent demonstration of anoxic conditions during starch accumulation in the endosperm. The specific late stage accumulation of the pyruvate-Pi-dikinase (PPDK) may suggest a critical role of this enzyme in the starch/protein balance through a PPi dependent restriction of ADPglucose synthesis, in addition to its usually reported influence on the alanine/aromatic amino acid synthesis balance.




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