Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on February 9, 2007; 10.1104/pp.106.092205

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
143/4/1853    most recent
pp.106.092205v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Svennerstam, H.
Right arrow Articles by Näsholm, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Svennerstam, H.
Right arrow Articles by Näsholm, T.
Agricola
Right arrow Articles by Svennerstam, H.
Right arrow Articles by Näsholm, T.

Received November 16, 2006
Accepted January 28, 2007

Comprehensive Screening of Arabidopsis Mutants Suggest the Lysine Histidine Transporter I to be Involved in Plant Uptake of Amino Acids

Henrik Svennerstam , Ulrika Ganeteg , Catherine Bellini , and Torgny Näsholm *

Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-901 83 Umea, Sweden; Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, route de St Cyr, 78026 Versailles Cedex, France

* Corresponding author; email: torgny.nasholm{at}genfys.slu.se.

Plant nitrogen (N) uptake is a key process in the global N cycle and is usually considered a "bottleneck" for biomass production in land ecosystems. Earlier, mineral N was considered the only form available to plants. Recent studies have questioned this dogma and shown that plants may access organic N sources such as amino acids. The actual mechanism enabling for plants to access amino acid N is still unknown. However, a recent study by Hirner et al. (2006) suggested the Lysine Histidine Transporter 1 (LHT1) to be involved in root amino acid uptake. In the present study, we isolated mutants defective in root amino acid uptake by screening Arabidopsis (Arabidopsis thaliana) seeds from ethyl methane sulfonate treated plants and seeds from amino acid transporter T-DNA knockout mutants for resistance against the toxic D-enantiomer of Alanine. Both ethyl methane sulfonate and T-DNA knockout plants identified as D-Ala resistant were found to be mutated in the LHT1 gene. LHT1 mutants displayed impaired capacity for uptake of a range of amino acids from solutions, displayed impaired growth when N was supplied in organic forms and acquired substantially lower amounts of amino acids than wild-type plants from solid growth media. LHT1 mutants grown on mineral N did not display a phenotype until at the stage of flowering, when premature senescence of old leaf pairs occurred suggesting that LHT1 may fulfill an important function at this developmental stage. Based on the broad and unbiased screening of mutants resistant to D-Ala, we suggest that LHT1 is an important mediator of root uptake of amino acids. This provides a molecular background for plant acquisition of organic N from the soil.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
N. Y. Komarova, K. Thor, A. Gubler, S. Meier, D. Dietrich, A. Weichert, M. Suter Grotemeyer, M. Tegeder, and D. Rentsch
AtPTR1 and AtPTR5 Transport Dipeptides in Planta
Plant Physiology, October 1, 2008; 148(2): 856 - 869.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Bertin, L. A. Weston, T. Huang, G. Jander, T. Owens, J. Meinwald, and F. C. Schroeder
From the Cover: Grass roots chemistry: meta-Tyrosine, an herbicidal nonprotein amino acid
PNAS, October 23, 2007; 104(43): 16964 - 16969.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists