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Published on January 5, 2007; 10.1104/pp.106.092817

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Received November 9, 2006
Accepted December 20, 2006

Major Proteome Variations Associated with Cherry Tomato Pericarp Development and Ripening

Mireille Faurobert *, Christina Mihr , Nadia Bertin , Tomasz Pawlowski , Luc Negroni , Nicolas Sommerer , and Mathilde Causse

INRA, Unité de Génétique et Amélioration des Fruits et Légumes, Domaine Saint-Maurice, BP94, 84143 Montfavet Cedex, France; INRA, Unité Plantes et Systèmes de culture Horticoles, Domaine Saint-Paul, 84914 Avignon Cedex 9, France; Instytut Dendrologii PAN 62-035 Kórnik, Poland; INRA, UMR de Génétique Végétale, IFR 87, Plate-Forme de Protéomique du Moulon, 91190 Gif-sur-Yvette, France; INRA, Unité de Recherches Protéomique, UR 1199, 34000 Montpellier, France

* Corresponding author; email: Mireille.Faurobert{at}avignon.inra.fr.

Tomato (Solanum lycopersicum) is a model plant for studying fleshy fruit development. Several genetic and molecular approaches have been developed to increase our knowledge about the physiological basis of fruit growth but very few data are yet available at the proteomic level. Main stages of fruit development were first determined through the dynamics of fruit diameter and pericarp cell number. Then, total proteins were extracted from pericarp tissue, at 6 relevant developmental stages, and separated by two dimensional gel electrophoresis. The protein patterns were markedly different between stages. The proteins showing major variations were monitored. We identified ninety of the 1791 well-resolved spots either by MALDI-TOF peptide mass fingerprinting or LC-MS/MS sequencing and EST database searching. Clustered correlation analysis results pointed out groups of proteins with similar expression profiles during fruit development. In young fruit, spots linked to amino acid metabolism or protein synthesis were mainly expressed during the cell division stage and down-regulated later. Some spots linked to cell division processes could be identified. During the cell expansion phase spots linked to photosynthesis and proteins linked to cell wall formation transiently increased. In contrast, the major part of the spots related to C-compounds and carbohydrate metabolism or oxidative processes were up-regulated during fruit development showing an increase in spot intensity during development and maximum abundance in mature fruit. This was also the case for the spots linked to stress responses and fruit senescence. We discuss the protein variations taking into account their potential role during fruit growth and comparing our results with already known variations at the mRNA and metabolite profiling levels.




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