Plant Physiology Preview
Published on January 26, 2007; 10.1104/pp.106.093005
Received November 14, 2006
Accepted January 8, 2007
Spatial Distribution and Temporal Variation of the Rice Si Transporter Lsi1
Naoki Yamaji and Jian Feng Ma *
Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan
* Corresponding author; email: maj{at}rib.okayama-u.ac.jp.
Rice is a typical Si accumulator and requires a large amount of Si for high yield production. Recently, a gene (Lsi1) encoding a Si transporter was identified in rice roots. Here, we characterized Lsi1 in terms of spatial distribution and temporal variation using both physiological and molecular approaches. Results from a multi-compartment transport-box experiment showed that the major site for Si uptake was located at the basal zone (>10 mm from the root tip) of the roots rather than at the root tips (<10 mm from the root tip). Consistent with the Si uptake pattern, Lsi1 expression and distribution of the Lsi1 protein were found only in the basal zone of roots. In the basal zones of the seminal, crown and lateral roots, the Lsi1 protein showed a polar localization at the distal side of both the exodermis and endodermis, where the Casparian bands are formed. This indicates that Lsi1 is required for the transport of Si through the cells of exodermis and endodermis. The expression of Lsi1 displayed a distinct diurnal pattern. Furthermore, the expression was transiently enhanced around the heading stage, which coincides with a high Si requirement during this growth stage. The expression was down-regulated by dehydration stress and ABA, suggesting that the expression of Lsi1 may be regulated by ABA.
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