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Published on February 23, 2007; 10.1104/pp.106.093088

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Received November 14, 2006
Accepted February 16, 2007

Characterisation of Two Brassinosteroid C-6 Oxidase Genes in Pea

Corinne E. Jager , Gregory M. Symons , Takahito Nomura , Yumiko Yamada , Jennifer J. Smith , Shinjiro Yamaguchi , Yuji Kamiya , James L. Weller , Takao Yokota , and James B. Reid *

School of Plant Science, University of Tasmania, Private Bag 55, Tasmania 7005, Australia; Plant Science Center, RIKEN, Yokohama 230-0045, Japan; Department of Biosciences, Teikyo University, Utsunomiya 320-8551

* Corresponding author; email: Jim.Reid{at}utas.edu.au.

C-6 oxidation genes play a key role in the regulation of biologically active brassinosteroid (BR) levels in the plant. They control BR activation, which involves the C-6 oxidation of 6-deoxocastasterone (6-DeoxoCS) to castasterone (CS) and in some cases the further conversion of CS to brassinolide (BL) . C-6 oxidation is controlled by the CYP85A family of P450s, and to date, two CYP85As have been isolated in tomato, two in Arabidopsis, one in rice, and one in grape. We have now isolated two CYP85As (CYP85A1 and CYP85A6) from pea (Pisum sativum L.). However, unlike Arabidopsis and tomato, which both contain one BR C-6 oxidase that converts 6-DeoxoCS to CS and one BR C-6 Baeyer-Villiger oxidase that converts 6-DeoxoCS right through to BL, the two BR C-6 oxidases in pea both act principally to convert 6-DeoxoCS to CS . The isolation of these two BR C-6 oxidation genes in pea highlights the species-specific differences associated with C-6 oxidation. In addition, we have isolated a novel BR-deficient mutant, lke, that blocks the function of one of these two BR C-6 oxidases (CYP85A6). The lke mutant exhibits a phenotype intermediate between wild-type plants and previously characterised pea BR mutants (lk, lka and lkb), and contains reduced levels of CS and increased levels of 6-DeoxoCS. To date, lke is the only mutant identified in pea that blocks the latter steps of BR biosynthesis and it will therefore provide an excellent tool to further examine the regulation of BR biosynthesis and the relative biological activities of CS and BL in pea.




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T. Nomura, M. Ueno, Y. Yamada, S. Takatsuto, Y. Takeuchi, and T. Yokota
Roles of Brassinosteroids and Related mRNAs in Pea Seed Growth and Germination
Plant Physiology, April 1, 2007; 143(4): 1680 - 1688.
[Abstract] [Full Text] [PDF]


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