Plant Physiology Preview
Published on March 16, 2007; 10.1104/pp.106.093997
OPEN ACCESS ARTICLE
Received December 1, 2006
Accepted March 6, 2007
Transcript Profiling of the Anoxic Rice Coleoptile
Rasika Lasanthi-Kudahettige , Leonardo Magneschi , Elena Loreti , Silvia Gonzali , Francesco Licausi , Giacomo Novi , Ottavio Beretta , Federico Vitulli , Amedeo Alpi , and Pierdomenico Perata *
Plant & Crop Physiology Laboratory, Scuola Superiore Sant'Anna, Via Mariscoglio 34, 56124 Pisa, Italy; IBBA-CNR, Via del Borghetto 80, 56100 Pisa, Italy; Department of Crop Plant Biology, University of Pisa, Via Mariscoglio 34, 56124 Pisa, Italy; Genopolis, University of Milano-Bicocca, Dept. of Biotechnology and Bioscience, Piazza della Scienza 4, 20126 Milano, Italy
* Corresponding author; email: p.perata{at}sssup.it.
Rice (Oryza sativa L.) seeds can germinate in complete absence of oxygen. Under anoxia, the rice coleoptile elongates, reaching a length greater than that of the aerobic one. In this paper, we compared and investigated the transcriptome of rice coleoptiles grown under aerobic and anaerobic conditions. The results allow drawing a detailed picture of the modulation of the transcripts involved in the anaerobic carbohydrate metabolism, suggesting the up-regulation of the steps required to produce and metabolize pyruvate and its derivatives. Sugars appear to play a signalling role under anoxia, with several genes indirectly up-regulated by anoxia-driven sugar starvation. The analysis of the effects of anoxia on the expansin gene families revealed that EXPA7 and EXPB12 are likely to be involved in the rice coleoptile elongation under anoxia. Genes coding for Ethylene Response Factors (ERFs) and Heat Shock Proteins are among the genes that are modulated by anoxia in both rice and Arabidopsis. The identification of anoxia-induced ERFs is suggestive, since genes belonging to this gene family play a crucial role in rice tolerance to submergence, a process closely related to, but independent from, the ability to germinate under anoxia. Genes coding for some enzymes requiring oxygen for their activity are dramatically down-regulated under anoxia, suggesting the existence of an energy-saving strategy in the regulation of gene expression.
This article has been cited by other articles:
|
|
|
|
 
D. Moldovan, A. Spriggs, J. Yang, B. J. Pogson, E. S. Dennis, and I. W. Wilson
Hypoxia-responsive microRNAs and trans-acting small interfering RNAs in Arabidopsis
J. Exp. Bot.,
October 8, 2009;
(2009)
erp296v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-W. Lee, P.-W. Chen, C.-A. Lu, S. Chen, T.-H. D. Ho, and S.-M. Yu
Coordinated Responses to Oxygen and Sugar Deficiency Allow Rice Seedlings to Tolerate Flooding
Sci. Signal.,
October 6, 2009;
2(91):
ra61 - ra61.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kant, Y.-M. Bi, T. Zhu, and S. J. Rothstein
SAUR39, a Small Auxin-Up RNA Gene, Acts as a Negative Regulator of Auxin Synthesis and Transport in Rice
Plant Physiology,
October 1, 2009;
151(2):
691 - 701.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Narsai, K. A. Howell, A. Carroll, A. Ivanova, A. H. Millar, and J. Whelan
Defining Core Metabolic and Transcriptomic Responses to Oxygen Availability in Rice Embryos and Young Seedlings
Plant Physiology,
September 1, 2009;
151(1):
306 - 322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Huang, N. L. Taylor, R. Narsai, H. Eubel, J. Whelan, and A. H. Millar
Experimental Analysis of the Rice Mitochondrial Proteome, Its Biogenesis, and Heterogeneity
Plant Physiology,
February 1, 2009;
149(2):
719 - 734.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Howell, R. Narsai, A. Carroll, A. Ivanova, M. Lohse, B. Usadel, A. H. Millar, and J. Whelan
Mapping Metabolic and Transcript Temporal Switches during Germination in Rice Highlights Specific Transcription Factors and the Role of RNA Instability in the Germination Process
Plant Physiology,
February 1, 2009;
149(2):
961 - 980.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. T. van Dongen, A. Frohlich, S. J. Ramirez-Aguilar, N. Schauer, A. R. Fernie, A. Erban, J. Kopka, J. Clark, A. Langer, and P. Geigenberger
Transcript and metabolite profiling of the adaptive response to mild decreases in oxygen concentration in the roots of arabidopsis plants
Ann. Bot.,
January 1, 2009;
103(2):
269 - 280.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Magneschi and P. Perata
Rice germination and seedling growth in the absence of oxygen
Ann. Bot.,
January 1, 2009;
103(2):
181 - 196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Ismail, E. S. Ella, G. V. Vergara, and D. J. Mackill
Mechanisms associated with tolerance to flooding during germination and early seedling growth in rice (Oryza sativa)
Ann. Bot.,
January 1, 2009;
103(2):
197 - 209.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kreuzwieser, J. Hauberg, K. A. Howell, A. Carroll, H. Rennenberg, A. H. Millar, and J. Whelan
Differential Response of Gray Poplar Leaves and Roots Underpins Stress Adaptation during Hypoxia
Plant Physiology,
January 1, 2009;
149(1):
461 - 473.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
