Plant Physiology Preview
Published on March 2, 2007; 10.1104/pp.106.094052
Received December 3, 2006
Accepted February 21, 2007
Actin Is Involved in Auxin-dependent Patterning
Jan Maisch * and Peter Nick
Institute of Botany 1, University of Karlsruhe, Kaiserstrasse 2, D-76128 Karlsruhe, Germany
* Corresponding author; email: jan.maisch{at}botanik1.uni-karlsruhe.de.
Polar transport of auxin has been identified as a central element of pattern formation. The polarity of auxin transport is linked to the cycling of pin-formed (PIN) proteins, a process that is related to actomyosin-dependent vesicle traffic. To get insight into the role of actin for auxin transport we used patterned cell division to monitor the polarity of auxin fluxes. We show that cell division in the tobacco (Nicotiana tabacum L. cv. Bright Yellow 2) cell line BY-2 is partially synchronized, and that this synchrony can be perturbed by inhibition of auxin transport by 1-N-naphthylphthalamic acid (NPA). To address the role of actin in this synchrony, we induced a bundled configuration of actin by overexpressing mouse talin (mT). The bundling of actin impairs the synchrony of cell division and increases the sensitivity to NPA. Addition of the polarly transported auxins indole-3-acetic acid and 1-naphthyl acetic acid (but not 2,4-dichlorophenoxyacetic acid) restored both the normal organization of actin and the synchrony of cell division. This study suggests that auxin controls its own transport by changing the state of actin filaments.
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