Plant Physiology Preview
Published on March 9, 2007; 10.1104/pp.106.094227
OPEN ACCESS ARTICLE
Received December 5, 2006
Accepted February 26, 2007
A Genomic Approach to Suberin Biosynthesis and Cork Differentiation
Marçal Soler , Olga Serra , Marisa Molinas , Gemma Huguet , Silvia Fluch , and Mercè Figueras *
Laboratori del suro, Department of Biology, Facultat de Ciències, Universitat de Girona, Campus Montilivi sn., 17071 Girona, Spain; PICME (Platform for Integrated Clone Management), ARC Austrian Research Center, A-2444 Seibersdorf
* Corresponding author; email: merce.figueras{at}udg.es.
Cork (phellem) is a multilayered dead tissue protecting plant mature stems and roots and plant healing tissues from water loss and injuries. Cork cells are made impervious by the deposition of suberin onto cell walls. Although suberin deposition and cork formation are essential for survival of land plants, molecular studies have rarely been conducted on this tissue. Here, we address this question by combining suppression subtractive hybridization (SSH) together with cDNA microarrays, using as a model the external bark of the cork-tree (Quercus suber), from which bottle cork is obtained. A SSH library from cork-tree bark was prepared containing 236 independent sequences; 69% showed significant homology to database sequences and they corresponded to 135 unique genes. Out of these genes, 43.5% were classified as the main pathways needed for cork biosynthesis. Furthermore, 19% could be related to regulatory functions. In order to identify genes more specifically required for suberin biosynthesis, cork ESTs were printed on a microarray and subsequently used to compare cork (phellem) to a non suberin producing tissue such as wood (xylem). Based on the results, a list of candidate genes relevant for cork was obtained. This list includes genes for the synthesis, transport and polymerization of suberin monomers such as components of the fatty acid elongase complexes, ABC transporters and acyltransferases, among others. Moreover, a number of regulatory genes induced in cork have been identified, including MYB, NAM and WRKY transcription factors with putative functions in meristem identity and cork differentiation.
This article has been cited by other articles:
|
|
|
|
 
I. Molina, Y. Li-Beisson, F. Beisson, J. B. Ohlrogge, and M. Pollard
Identification of an Arabidopsis Feruloyl-Coenzyme A Transferase Required for Suberin Synthesis
Plant Physiology,
November 1, 2009;
151(3):
1317 - 1328.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Compagnon, P. Diehl, I. Benveniste, D. Meyer, H. Schaller, L. Schreiber, R. Franke, and F. Pinot
CYP86B1 Is Required for Very Long Chain {omega}-Hydroxyacid and {alpha},{omega}-Dicarboxylic Acid Synthesis in Root and Seed Suberin Polyester
Plant Physiology,
August 1, 2009;
150(4):
1831 - 1843.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Serra, M. Soler, C. Hohn, R. Franke, L. Schreiber, S. Prat, M. Molinas, and M. Figueras
Silencing of StKCS6 in potato periderm leads to reduced chain lengths of suberin and wax compounds and increased peridermal transpiration
J. Exp. Bot.,
February 1, 2009;
60(2):
697 - 707.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Serra, M. Soler, C. Hohn, V. Sauveplane, F. Pinot, R. Franke, L. Schreiber, S. Prat, M. Molinas, and M. Figueras
CYP86A33-Targeted Gene Silencing in Potato Tuber Alters Suberin Composition, Distorts Suberin Lamellae, and Impairs the Periderm's Water Barrier Function
Plant Physiology,
February 1, 2009;
149(2):
1050 - 1060.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Hofer, I. Briesen, M. Beck, F. Pinot, L. Schreiber, and R. Franke
The Arabidopsis cytochrome P450 CYP86A1 encodes a fatty acid {omega}-hydroxylase involved in suberin monomer biosynthesis
J. Exp. Bot.,
June 1, 2008;
59(9):
2347 - 2360.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Luo, X.-Y. Xue, W.-L. Hu, L.-J. Wang, and X.-Y. Chen
An ABC Transporter Gene of Arabidopsis thaliana, AtWBC11, is Involved in Cuticle Development and Prevention of Organ Fusion
Plant Cell Physiol.,
December 1, 2007;
48(12):
1790 - 1802.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, F. Beisson, A. J. K. Koo, I. Molina, M. Pollard, and J. Ohlrogge
Identification of acyltransferases required for cutin biosynthesis and production of cutin with suberin-like monomers
PNAS,
November 13, 2007;
104(46):
18339 - 18344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
