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Published on June 7, 2007; 10.1104/pp.106.094946

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Received December 20, 2006
Accepted June 4, 2007

Exclusion of Na+ via a Sodium ATPase (PpENA1) Ensures Normal Growth of Physcomitrella patens under Moderate Salt Stress

Christina Lunde *, Damian P. Drew , Andrew K. Jacobs , and Mark Tester

Plant Biochemistry Laboratory, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Copenhagen, Denmark; Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, SA, 5064, Australia

* Corresponding author; email: chlu{at}life.ku.dk.

The bryophyte Physcomitrella patens is unlike any other plant identified to date in that it possesses a gene which encodes an ENA-type Na+-ATPase (Benito and Rodriguez-Navarro, 2003). To complement previous work in yeast, we determined the importance of having a Na+-ATPase in planta by conducting physiological analyses of PpENA1 in Physcomitrella. Expression studies showed that PpENA1 is upregulated by NaCl and to a lesser degree by osmotic stress. Maximal induction is obtained after 8 hours at 60 mM NaCl or above. No other abiotic stress tested led to significant increases in PpENA1 expression. In the gametophyte strong expression was confined to the rhizoids, stem and the basal part of the leaf. In the protonemata, expression was ubiquitous with a few filaments showing stronger expression. At 100 mM NaCl wild type plants were able to maintain a higher K+/Na+ ratio than the PpENA1 (ena1) gene knockout, but at higher NaCl concentrations no difference was observed. Although no difference in chlorophyll content was observed between ena1 and wild type at 100 mM NaCl, the impaired Na+ exclusion in ena1 plants led to a ~40% decrease in growth.




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