Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 QUICK SEARCH:   [advanced]


     


Plant Physiology Preview
Published on March 9, 2007; 10.1104/pp.106.094979


OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
144/1/105    most recent
pp.106.094979v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ramirez-Parra, E.
Right arrow Articles by Gutierrez, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramirez-Parra, E.
Right arrow Articles by Gutierrez, C.
Agricola
Right arrow Articles by Ramirez-Parra, E.
Right arrow Articles by Gutierrez, C.

Received December 18, 2006
Accepted March 1, 2007

E2F Regulates FASCIATA1, a Chromatin Assembly Gene whose Loss Switches on the Endocycle and Activates Gene Expression by Changing the Epigenetic Status

Elena Ramirez-Parra and Crisanto Gutierrez *

Centro de Biología Molecular "Severo Ochoa", Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco 28049, Madrid, Spain

* Corresponding author; email: cgutierrez{at}cbm.uam.es.

Maintenance of genome integrity depends on histone chaperone-mediated chromatin reorganization. DNA replication-associated nucleosome deposition relies on the chromatin assembly factor 1 (CAF-1). Depletion of CAF-1 in human cells leads to cell death whereas in Arabidopsis, where it is involved in heterochromatin compaction and homologous recombination, plants are viable. The mechanism that makes the lack of CAF-1 activity compatible with development is not known. Here, we show that the FASCIATA1 (FAS1) gene, which encodes the CAF-1 large subunit, is a target of the E2F transcription factors. Mutational studies demonstrate that one of the two E2F binding sites in its promoters has an activator role while the other has a repressor function. Loss of FAS1 results in reduced CDKA activity, inhibits mitotic progression and promotes a precocious and systemic switch to the endocycle program. A selective up-regulation of the expression of a subset of genes, including those involved in activation of the G2 DNA damage checkpoint, also occurs upon FAS1 loss. This activation is not the result of a global change in chromatin structure but depends on selective epigenetic changes in histone acetylation and methylation within a small region in their promoters. This suggests that a correct chromatin assembly during S-phase is required to prevent unscheduled changes in the epigenetic marks of target genes. Interestingly, activation of the endocycle switch as well as introduction of activating histone marks in the same set of G2 checkpoint genes is detected upon treatment of wild type plants with DNA damaging treatments. Our results are consistent with a model in which defects in chromatin assembly during S-phase and the DNA damage signaling share part of a pathway, which ultimately lead to mitotic arrest and triggers the endocycle program. Together, this might be a bypass mechanism that makes development compatible with cell division arrest induced by DNA damage stress.




This article has been cited by other articles:


Home page
J Exp BotHome page
J. M. Plett, J. Mathur, and S. Regan
Ethylene receptor ETR2 controls trichome branching by regulating microtubule assembly in Arabidopsis thaliana
J. Exp. Bot., September 1, 2009; 60(13): 3923 - 3933.
[Abstract] [Full Text] [PDF]


Home page
Plant Physiol.Home page
H. Roa, J. Lang, K. M. Culligan, M. Keller, S. Holec, V. Cognat, M.-H. Montane, G. Houlne, and M.-E. Chaboute
Ribonucleotide Reductase Regulation in Response to Genotoxic Stress in Arabidopsis
Plant Physiology, September 1, 2009; 151(1): 461 - 471.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
H. Yoshikawa, K. Morishima, T. Fujimoto, T. Saito, T. Kobayashi, E. Yamaha, and K. Arai
Chromosome Doubling in Early Spermatogonia Produces Diploid Spermatozoa in a Natural Clonal Fish
Biol Reprod, May 1, 2009; 80(5): 973 - 979.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
M. de la Paz Sanchez and C. Gutierrez
From the Cover: Arabidopsis ORC1 is a PHD-containing H3K4me3 effector that regulates transcription
PNAS, February 10, 2009; 106(6): 2065 - 2070.
[Abstract] [Full Text] [PDF]


Home page
Plant CellHome page
H. Yin, X. Zhang, J. Liu, Y. Wang, J. He, T. Yang, X. Hong, Q. Yang, and Z. Gong
Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase {epsilon} Mutation in Arabidopsis
PLANT CELL, February 1, 2009; 21(2): 386 - 402.
[Abstract] [Full Text] [PDF]


Home page
Cold Spring Harb Symp Quant BiolHome page
N. Kornet and B. Scheres
Stem Cell Factors in Plants: Chromatin Connections
Cold Spring Harb Symp Quant Biol, January 15, 2009; (2009) sqb.2008.73.043v1.
[Abstract] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
T. Lammens, V. Boudolf, L. Kheibarshekan, L. Panagiotis Zalmas, T. Gaamouche, S. Maes, M. Vanstraelen, E. Kondorosi, N. B. La Thangue, W. Govaerts, et al.
Atypical E2F activity restrains APC/CCCS52A2 function obligatory for endocycle onset
PNAS, September 23, 2008; 105(38): 14721 - 14726.
[Abstract] [Full Text] [PDF]


Home page
Plant Physiol.Home page
J. T. Ascencio-Ibanez, R. Sozzani, T.-J. Lee, T.-M. Chu, R. D. Wolfinger, R. Cella, and L. Hanley-Bowdoin
Global Analysis of Arabidopsis Gene Expression Uncovers a Complex Array of Changes Impacting Pathogen Response and Cell Cycle during Geminivirus Infection
Plant Physiology, September 1, 2008; 148(1): 436 - 454.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
Z. Chen, J. L. H. Tan, M. Ingouff, V. Sundaresan, and F. Berger
Chromatin assembly factor 1 regulates the cell cycle but not cell fate during male gametogenesis in Arabidopsis thaliana
Development, January 1, 2008; 135(1): 65 - 73.
[Abstract] [Full Text] [PDF]


Home page
Plant CellHome page
C. Breuer, N. J. Stacey, C. E. West, Y. Zhao, J. Chory, H. Tsukaya, Y. Azumi, A. Maxwell, K. Roberts, and K. Sugimoto-Shirasu
BIN4, a Novel Component of the Plant DNA Topoisomerase VI Complex, Is Required for Endoreduplication in Arabidopsis
PLANT CELL, November 1, 2007; 19(11): 3655 - 3668.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists