Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on March 16, 2007; 10.1104/pp.106.095281

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
144/1/503    most recent
pp.106.095281v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Parre, E.
Right arrow Articles by Savouré, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parre, E.
Right arrow Articles by Savouré, A.
Agricola
Right arrow Articles by Parre, E.
Right arrow Articles by Savouré, A.

Received December 26, 2006
Accepted March 13, 2007

Calcium Signaling Via Phospholipase C Is Essential for Proline Accumulation upon Ionic but not Non-ionic Hyperosmotic Stresses in Arabidopsis thaliana

Elodie Parre , Mohamed Ali Ghars , Anne-Sophie Leprince , Laurent Thiery , Delphine Lefebvre , Marianne Bordenave , Luc Richard , Christian Mazars , Chedly Abdelly , and Arnould Savouré *

Université Pierre et Marie Curie-Paris6, UMR 7180, PCMP, Case 156, 4 place Jussieu, Paris, F-75005 France ; CNRS, UMR 7180, PCMP, Case 156, 4 place Jussieu, Paris, F-75005 France; INRST, Adaptation des Plantes aux Stress Abiotiques, Hamman lif 2050, Tunisia; Université Paul Sabatier, UMR CNRS-UPS 5546, 24 chemin de Borde Rouge, BP 42617, Auzeville, 31326 Castanet-Tolosan, France

* Corresponding author; email: savoure{at}ccr.jussieu.fr.

Proline accumulation occurs in various plant organisms in response to environmental stresses. To identify the signaling components involved in the regulation of proline metabolism upon water stress in Arabidopsis thaliana, a pharmacological approach was developed. The role of phosphoinositide-specific phospholipases C (PLC) in proline accumulation was assessed by the use of the aminosteroid U73122, a commonly employed specific inhibitor of receptor-mediated PLCs. We found that U73122 reduced pyrroline-5-carboxylate synthetase transcript and protein as well as proline levels in salt-treated seedlings. Inhibition of PLC activity by U73122 was quantified by measuring the decrease of inositol 1,4,5-trisphosphate (InsP3) levels. Moreover, the utilization of diacylglycerol kinase and InsP3-gated calcium release receptor inhibitors suggested that InsP3 or its derivatives are essential for proline accumulation upon salt stress, involving calcium as a second messenger in ionic stress signaling. This observation was further supported by a partial restoration of proline accumulation in salt- and U73122-treated seedlings after addition of extracellular calcium, or when calcium homeostasis was perturbed by cyclopiazonic acid (CPA), a blocker of plant type IIA calcium pumps. Taken together, our data indicate that PLC-based signaling is a committed step in proline biosynthesis upon salinity but not in the case of mannitol stress. Calcium acts as a molecular switch to trigger downstream signaling events. These results also demonstrated the specific involvement of lipid signaling pathway to discriminate between ionic and non-ionic stresses.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
X. Ma, O. Shor, S. Diminshtein, L. Yu, Y. J. Im, I. Perera, A. Lomax, W. F. Boss, and N. Moran
Phosphatidylinositol (4,5)Bisphosphate Inhibits K+-Efflux Channel Activity in NT1 Tobacco Cultured Cells
Plant Physiology, February 1, 2009; 149(2): 1127 - 1140.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. L. Lanteri, A. M. Laxalt, and L. Lamattina
Nitric Oxide Triggers Phosphatidic Acid Accumulation via Phospholipase D during Auxin-Induced Adventitious Root Formation in Cucumber
Plant Physiology, May 1, 2008; 147(1): 188 - 198.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Alhagdow, F. Mounet, L. Gilbert, A. Nunes-Nesi, V. Garcia, D. Just, J. Petit, B. Beauvoit, A. R. Fernie, C. Rothan, et al.
Silencing of the Mitochondrial Ascorbate Synthesizing Enzyme L-Galactono-1,4-Lactone Dehydrogenase Affects Plant and Fruit Development in Tomato
Plant Physiology, December 1, 2007; 145(4): 1408 - 1422.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists