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Published on March 16, 2007; 10.1104/pp.106.095281

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Received December 26, 2006
Accepted March 13, 2007

Calcium Signaling Via Phospholipase C Is Essential for Proline Accumulation upon Ionic but not Non-ionic Hyperosmotic Stresses in Arabidopsis thaliana

Elodie Parre , Mohamed Ali Ghars , Anne-Sophie Leprince , Laurent Thiery , Delphine Lefebvre , Marianne Bordenave , Luc Richard , Christian Mazars , Chedly Abdelly , and Arnould Savouré *

Université Pierre et Marie Curie-Paris6, UMR 7180, PCMP, Case 156, 4 place Jussieu, Paris, F-75005 France ; CNRS, UMR 7180, PCMP, Case 156, 4 place Jussieu, Paris, F-75005 France; INRST, Adaptation des Plantes aux Stress Abiotiques, Hamman lif 2050, Tunisia; Université Paul Sabatier, UMR CNRS-UPS 5546, 24 chemin de Borde Rouge, BP 42617, Auzeville, 31326 Castanet-Tolosan, France

* Corresponding author; email: savoure{at}ccr.jussieu.fr.

Proline accumulation occurs in various plant organisms in response to environmental stresses. To identify the signaling components involved in the regulation of proline metabolism upon water stress in Arabidopsis thaliana, a pharmacological approach was developed. The role of phosphoinositide-specific phospholipases C (PLC) in proline accumulation was assessed by the use of the aminosteroid U73122, a commonly employed specific inhibitor of receptor-mediated PLCs. We found that U73122 reduced pyrroline-5-carboxylate synthetase transcript and protein as well as proline levels in salt-treated seedlings. Inhibition of PLC activity by U73122 was quantified by measuring the decrease of inositol 1,4,5-trisphosphate (InsP3) levels. Moreover, the utilization of diacylglycerol kinase and InsP3-gated calcium release receptor inhibitors suggested that InsP3 or its derivatives are essential for proline accumulation upon salt stress, involving calcium as a second messenger in ionic stress signaling. This observation was further supported by a partial restoration of proline accumulation in salt- and U73122-treated seedlings after addition of extracellular calcium, or when calcium homeostasis was perturbed by cyclopiazonic acid (CPA), a blocker of plant type IIA calcium pumps. Taken together, our data indicate that PLC-based signaling is a committed step in proline biosynthesis upon salinity but not in the case of mannitol stress. Calcium acts as a molecular switch to trigger downstream signaling events. These results also demonstrated the specific involvement of lipid signaling pathway to discriminate between ionic and non-ionic stresses.




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