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PLANTS INTERACTING WITH OTHER ORGANISMS

Magnaporthe grisea Infection Triggers RNA Variation and Antisense Transcript Expression in Rice^1,[W]

Department of Plant Pathology (M.G., R.-C.V., H.L., C.J., G.-L.W.) and Ohio Supercomputer Center (E.S.), Ohio State University, Columbus, Ohio 43212; Arizona Genomics Computational Laboratory, BIO5 Institute (V.P., C.S.) and Arizona Genomics Institute and Department of Plant Sciences (H.K., R.W.), University of Arizona, Tucson, Arizona 85721; Fungal Genomics Laboratory, Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695 (R.A.D.); and Rice Genomics Laboratory, Hunan Agricultural University, Changsha, Hunan 410128, China (G.-L.W.)

Rice blast disease, caused by the fungal pathogen Magnaporthe grisea, is an excellent model system to study plant-fungal interactions and host defense responses. In this study, comprehensive analysis of the rice (Oryza sativa) transcriptome after M. grisea infection was conducted using robust-long serial analysis of gene expression. A total of 83,382 distinct 21-bp robust-long serial analysis of gene expression tags were identified from 627,262 individual tags isolated from the resistant (R), susceptible (S), and control (C) libraries. Sequence analysis revealed that the tags in the R and S libraries had a significant reduced matching rate to the rice genomic and expressed sequences in comparison to the C library. The high level of one-nucleotide mismatches of the R and S library tags was due to nucleotide conversions. The A-to-G and U-to-C nucleotide conversions were the most predominant types, which were induced in the M. grisea-infected plants. Reverse transcription-polymerase chain reaction analysis showed that expression of the adenine deaminase and cytidine deaminase genes was highly induced after inoculation. In addition, many antisense transcripts were induced in infected plants and expression of four antisense transcripts was confirmed by strand-specific reverse transcription-polymerase chain reaction. These results demonstrate that there is a series of dynamic and complex transcript modifications and changes in the rice transcriptome at the M. grisea early infection stages.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Guo-Liang Wang (wang.620{at}osu.edu).

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.095653

^* Corresponding author; e-mail wang.620{at}osu.edu; fax 614–292–4455.

Received January 8, 2007; accepted February 15, 2007; published March 9, 2007.