Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on March 9, 2007; 10.1104/pp.107.096131

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
144/1/94    most recent
pp.107.096131v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Salmi, M. L.
Right arrow Articles by Porterfield, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salmi, M. L.
Right arrow Articles by Porterfield, D. M.
Agricola
Right arrow Articles by Salmi, M. L.
Right arrow Articles by Porterfield, D. M.

Received January 18, 2007
Accepted February 27, 2007

Nitric Oxide and cGMP Signaling in Calcium-Dependent Development of Cell Polarity in Ceratopteris richardii

Mari L. Salmi , Kacey E. Morris , Stanley J. Roux , and D. Marshall Porterfield *

Section of Molecular Cell and Developmental Biology, University of Texas at Austin, Austin, TX 78712; Department of Biological Sciences, University of Missouri-Rolla, Rolla, MO, 65409; Department of Agricultural & Biological Engineering and Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907-2093

* Corresponding author; email: porterf{at}purdue.edu.

Single-celled spores of the fern Ceratopteris richardii undergo gravity-directed cell polarity development that is driven by polar calcium currents. Here we present results that establish a role for NO/cGMP signaling in transducing the stimulus of gravity to directed polarization of the spores. Application of specific NO donors and scavengers inhibited the calcium-dependent gravity response in a dose-dependent manner. The effects of NO donor exposure were antagonized by application of NO scavenger compounds. Similarly, the guanylate cyclase inhibitors LY83583 and ODQ, and the phosphodiesterase inhibitor Viagra®, which modulate NO-dependent cGMP levels in the cells, disrupted gravity-directed cell polarity in a dose-dependent manner. Viagra® effects were antagonized by application of NO scavengers, consistent with the postulate that NO and cGMP are linked in the signaling pathway. To identify other components of the signaling system we analyzed gene expression changes induced by Viagra® treatment using microarrays and quantitative real-time RT-PCR (Q RT-PCR). Preliminary microarray analysis revealed several genes whose expression was significantly altered by Viagra® treatment. Three of these genes had strong sequence similarity to key signal transduction or stress response genes, and Q RT-PCR was used to more rigorously quantify the effects of Viagra® on their expression in spores and to test how closely these effects could be mimicked by treatment with dibutyryl cGMP. Taken together our, results implicate NO and cGMP as downstream effectors that help link the gravity stimulus to polarized growth in C. richardii spores.




This article has been cited by other articles:


Home page
 J Exp BotHome page
A. Kasprowicz, A. Szuba, D. Volkmann, F. Baluska, and P. Wojtaszek
Nitric oxide modulates dynamic actin cytoskeleton and vesicle trafficking in a cell type-specific manner in root apices
J. Exp. Bot., April 1, 2009; 60(6): 1605 - 1617.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists