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Published on June 15, 2007; 10.1104/pp.107.096172

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Received January 28, 2007
Accepted June 8, 2007

Novel Insights into Seed Fatty Acid Synthesis and Modification Pathways from Genetic Diversity and QTL Analysis of the Brassica C genome

Guy C. Barker *, Tony R. Larson , Ian A. Graham , James R. Lynn , and Graham J. King

Warwick HRI, University of Warwick, Wellesbourne, Warwick, CV35 9EF, UK; CNAP, Department of Biology, University of York, PO Box 373, York YO10 5YW, UK; Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK

* Corresponding author; email: Guy.Barker{at}warwick.ac.uk.

Natural genetic variation in fatty acid synthesis and modification pathways determine the composition of vegetable oils, which are major components of human diet and renewable products. Based on known pathways we combined diversity and genetic analysis of metabolites to infer the existence of enzymes encoded by distinct loci, and associated these with specific elongation steps or sub-pathways. 107 lines representing different Brassica genepools revealed considerable variation for 18 seed fatty acid products. The effect of genetic variation within a single biochemical step on subsequent products was demonstrated using a correlation matrix of scatterplots, and by calculating relative step yields. Surprisingly, diploid B. oleracea segregating populations had a similar range of variation for individual fatty acids as across the whole genepool. This allowed identification of 22 QTL associated with activity in the plastid, early stages of synthesis, desaturation and elongases. Four QTL were assigned to early stages of synthesis, seven to sub-pathway specific or general elongase activity, one to KAS, two each to FAD and either desaturase or FAT. An additional 10 QTL had distinct effects but were not assigned specific functions. Where contrasting behaviour in more than one subpathway was detected, we inferred QTL specificity for particular combinations of substrate and product. The assignment of enzyme function to QTL was consistent with the known position of some Brassicaeae candidate genes and collinear regions of the Arabidopsis genome.




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