Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on June 7, 2007; 10.1104/pp.107.096347

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
144/4/1960    most recent
pp.107.096347v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Phan, T. D.
Right arrow Articles by Tucker, G. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Phan, T. D.
Right arrow Articles by Tucker, G. A.
Agricola
Right arrow Articles by Phan, T. D.
Right arrow Articles by Tucker, G. A.

Received January 24, 2007
Accepted May 19, 2007

Silencing of the Major Salt-dependent Isoform of Pectinesterase in Tomato Alters Fruit Softening

Thanh D. Phan , Wen. Bo , Gill. West , Grantley W. Lycett , and Gregory A. Tucker *

University of Nottingham, School of Biosciences, Sutton Bonington Campus, Loughborough, Leics. LE12 5RD, United Kingdom

* Corresponding author; email: gregory.tucker{at}nottingham.ac.uk.

Pectinesterase (E.C 3.1.1.11) (PE) is an enzyme responsible for the de-methylation of galacturonyl residues in high molecular weight pectin; and is believed to play an important role in cell wall metabolism. In this study, Pmeu1, a ubiquitously expressed PE gene has been characterised by anti-sense suppression in tomato (Solanum lycopersicum). Transgenic tomato plants showed reduced PE activity levels in both green fruit and leaf tissue to around 65% and 25% of that found in wild type plants, respectively. Pmeu1 was observed to encode a salt dependent PE isoform that correlated with PE1 as previously described in fruit tissue. Silencing of Pmeu1 did not result in any detectable phenotype within the leaf tissue despite the gene product representing the major isoform in this tissue. In comparison silencing in fruit resulted in an enhancement to the rate of softening during ripening. The role of PMEU1 in fruit ripening is discussed.




This article has been cited by other articles:


Home page
 J Exp BotHome page
G. Lycett
The role of Rab GTPases in cell wall metabolism
J. Exp. Bot., November 1, 2008; 59(15): 4061 - 4074.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists