Plant Physiology Preview
Published on March 16, 2007; 10.1104/pp.107.096586
Received January 25, 2007
Accepted March 8, 2007
The Rice YAB1 Gene Is Involved in the Feedback Regulation of Gibberellin Metabolism
Mingqiu Dai , Yu Zhao , Qian Ma , Yongfeng Hu , Peter Hedden , Qifa Zhang , and Dao-Xiu Zhou *
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Institut de Biotechnologie des Plantes, Université Paris sud 11, Orsay, F-91405, France; Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK
* Corresponding author; email: dao-xiu.zhou{at}u-psud.fr.
Gibberellin (GA) biosynthesis is regulated by feedback control providing a mechanism for GA homeostasis in plants. However, regulatory elements involved in the feedback control are not known. In this report, we show that a rice YABBY gene (YAB1) had a similar expression pattern as key rice GA biosynthetic genes GA3ox2 and GA20ox2. Over-expression of YAB1 in transgenic rice resulted in a semi-dwarf phenotype that could be fully rescued by applied GA. Quantification of the endogenous GA content revealed increases of GA20 and decreases of GA1 levels in the over-expression plants, in which the transcripts of the biosynthetic gene GA3ox2 were decreased. Co-suppression of YAB1 in transgenic plants induced expression of GA3ox2. The repression of GA3ox2 could be obtained upon treatment by dexamethasone of transgenic plants expressing a YAB1-GR fusion. Importantly, we show that YAB1 bound to a GA-responsive element (GARE) within the GA3ox2 promoter. In addition, the expression of YAB1 was deregulated in GA biosynthesis and signalling mutants and could be either transiently induced by GA or repressed by a GA inhibitor. Finally, either over-expression or co-suppression of YAB1 impaired GA-mediated repression of GA3ox2. These data together suggest that YAB1 is involved in the feedback regulation of GA biosynthesis in rice.
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