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Published on June 15, 2007; 10.1104/pp.107.097584

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Received February 7, 2007
Accepted May 24, 2007

Impacts of T-Phylloplanin Gene Knockdown and of Helianthus and Datura Phylloplanins on Peronospora tabicina Spore Germination and Disease potential

Antoaneta B. Kroumova , Ryan W. Shepherd , and George J. Wagner *

Plant Biology Program, Plant and Soil Sciences Department, University of Kentucky, Lexington, KY 40546; Plant and Microbial Biology Department, University of California-Berkeley, Berkeley, CA 94720

* Corresponding author; email: gwagner{at}uky.edu.

T-phylloplanin proteins secreted to aerial surfaces of Nicotiana tabacum by short procumbent trichomes inhibit spore germination and blue mold disease caused by the oomycete pathogen Peronospora tabacina. Many other plants were found to contain water washed leaf surface proteins (phylloplanins), but the functions and properties of these are not known. Here we extend earlier evidence (Plant Cell 17:1851-1861, 2005) for the antifungal activity of T-phylloplanins using a reverse genetics approach. RNAi of the T-phylloplanin gene in Nicotiana tabacum, c.v. T.I. 1068 resulted in loss of T-phylloplanin mRNA and protein, loss of in vitro spore germination inhibition activity and leaf infection inhibition activity of leaf water washes from RNAi plants, and young knockdown plants were susceptible to disease. The glycoprotein character, adaxial-leaf-surface enrichment of, and renew-ability of T-phylloplanins are also described. We also report that leaf water washes of Helianthus annuus and Datura metel, but not Glycine max, like that of N. tabacum, possess ProteinaseK- and boiling-sensitive P. tabacina spore germination and tobacco leaf infection inhibition activities. Results establish that T-phylloplaninins of N. tabacum are active in P. tabacina inhibition, and indicate that leaf surface proteins of certain non-Nicotiana species that are not susceptible to P. tabacina disease can inhibit germination of spores of this oomycete pathogen and inhibit tobacco leaf infection by this pathogen.




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