Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on April 27, 2007; 10.1104/pp.107.097980

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
144/3/1455    most recent
pp.107.097980v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Siciliano, V.
Right arrow Articles by Bonfante, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Siciliano, V.
Right arrow Articles by Bonfante, P.
Agricola
Right arrow Articles by Siciliano, V.
Right arrow Articles by Bonfante, P.

Received February 13, 2007
Accepted April 13, 2007

Transcriptome Analysis of Arbuscular Mycorrhizal Roots during Development of the Pre-penetration Apparatus

Valeria Siciliano , Andrea Genre , Raffaella Balestrini , Gilda Cappellazzo , Pierre J.G.M. deWit , and Paola Bonfante *

Dipartimento di Biologia Vegetale, Università di Torino and IPP-CNR. Viale Mattioli 25 - 10125 Torino, Italy; Laboratory of Phytopathology, Wageningen University, Binnenhaven 5 - 6709 PD Wageningen, The Netherlands

* Corresponding author; email: p.bonfante{at}ipp.cnr.it.

Information on changes in the plant transcriptome during early interaction with arbuscular mycorrhizal (AM) fungi is still limited since infections are usually not synchronized and plant markers for early stages of colonization are not yet available. A pre-penetration apparatus (PPA), organized in epidermal cells during appressorium development, has reported to be responsible for assembling a trans-cellular tunnel to accommodate the invading fungus (Genre et al., 2005). Here we used PPAs as markers for cell responsiveness to fungal contact in order to investigate gene expression at this early stage of infection with minimal transcript dilution. PPAs were identified by confocal microscopy in transformed roots of Medicago truncatula expressing GFP:HDEL, colonized by the AM fungus Gigaspora margarita. A PPA-targeted suppressive-subtractive cDNA library was built, the cDNAs were cloned and sequenced, and led to the identification of 107 putative interaction-specific genes. The expression of a subset of 15 genes, selected by reverse Northern dot blot screening, and five additional genes, potentially involved in PPA formation, was analyzed by real-time RT PCR and compared with an infection stage, 48 hours after the onset of the PPA. Comparison of the expression profile of G. margarita-inoculated wild-type (WT) and the mycorrhiza-defective dmi3-1 mutant of M. truncatula revealed that an expansin-like gene, expressed in WT epidermis during PPA development, can be regarded as an early host marker for successful mycorrhization. A putative Avr9/Cf9 rapidly elicited gene, found to be up-regulated in the mutant, suggests novel regulatory roles for the DMI3 protein in the early mycorrhization process.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. Genre, G. Ortu, C. Bertoldo, E. Martino, and P. Bonfante
Biotic and Abiotic Stimulation of Root Epidermal Cells Reveals Common and Specific Responses to Arbuscular Mycorrhizal Fungi
Plant Physiology, March 1, 2009; 149(3): 1424 - 1434.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Gutjahr, M. Banba, V. Croset, K. An, A. Miyao, G. An, H. Hirochika, H. Imaizumi-Anraku, and U. Paszkowski
Arbuscular Mycorrhiza-Specific Signaling in Rice Transcends the Common Symbiosis Signaling Pathway
PLANT CELL, November 1, 2008; 20(11): 2989 - 3005.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists