Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on May 11, 2007; 10.1104/pp.107.098152

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrowOA All Versions of this Article:
144/3/1316    most recent
pp.107.098152v2
pp.107.098152v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tetu, S. G.
Right arrow Articles by Ludwig, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tetu, S. G.
Right arrow Articles by Ludwig, M.
Agricola
Right arrow Articles by Tetu, S. G.
Right arrow Articles by Ludwig, M.

Received February 19, 2007
Accepted May 4, 2007

The Flaveria bidentis beta-carbonic Anhydrase Gene Family Encodes Cytosolic and Chloroplastic Isoforms Demonstrating Distinct Organ-specific Expression Patterns

Sasha G. Tetu , Sandra K. Tanz , Nicole Vella , James N. Burnell , and Martha Ludwig *

Department of Biological Sciences, Macquarie University, Sydney, New South Wales, 2109, Australia; Department of Biochemistry and Molecular Biology, James Cook University, Townsville, Queensland, 4811, Australia; School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, Crawley, Western Australia, 6009, Australia

* Corresponding author; email: mludwig{at}cyllene.uwa.edu.au.

Carbonic anhydrase (CA) catalyzes the interconversion of CO2 and bicarbonate, the forms of inorganic carbon used by the primary carboxylating enzymes of C3 and C4 plants, respectively. Multiple forms of CA are found in both photosynthetic subtypes; however, the number of isoforms and the location and function of each have not been elucidated for any single plant species. Genomic Southern analyses showed the C4 dicotyledon Flaveria bidentis (L.) Kuntze contains a small gene family encoding {beta}-CA, and cDNAs encoding three distinct {beta}-CAs, named CA1, CA2 and CA3, were isolated. Quantitative reverse transcription-PCR showed each member of this {beta}-CA family has a specific expression pattern in F. bidentis leaves, roots and flowers. CA3 transcript levels were at least 50-times more abundant than CA2 or CA1 transcripts in leaves. CA2 transcripts were detected in all organs examined and were the most abundant CA transcripts in roots. CA1 mRNA levels were similar to those of CA2 in leaves, but were considerably lower in roots and flowers. In vitro import assays showed CA1 was imported into isolated pea chloroplasts while CA2 and CA3 were not. These results support the following roles for F. bidentis CAs: CA3 is responsible for catalyzing the first step in the C4 pathway in the mesophyll cell cytosol; CA2 provides bicarbonate for anapleurotic reactions involving non-photosynthetic forms of phosphoenolpyruvate carboxylase in the cytosol of cells in both photosynthetic and non-green tissues; and CA1 carries out non-photosynthetic functions demonstrated by C3 chloroplastic {beta}-CAs, including lipid biosynthesis and antioxidant activity.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S. K. Tanz, S. G. Tetu, N. G.F. Vella, and M. Ludwig
Loss of the Transit Peptide and an Increase in Gene Expression of an Ancestral Chloroplastic Carbonic Anhydrase Were Instrumental in the Evolution of the Cytosolic C4 Carbonic Anhydrase in Flaveria
Plant Physiology, July 1, 2009; 150(3): 1515 - 1529.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Hendrickson, R. Sharwood, M. Ludwig, S. M. Whitney, M. R. Badger, and S. von Caemmerer
The effects of Rubisco activase on C4 photosynthesis and metabolism at high temperature
J. Exp. Bot., May 1, 2008; 59(7): 1789 - 1798.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists