Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on May 11, 2007; 10.1104/pp.107.098681

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
144/3/1256    most recent
pp.107.098681v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kris, R. M.
Right arrow Articles by Galbraith, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kris, R. M.
Right arrow Articles by Galbraith, D. W.
Agricola
Right arrow Articles by Kris, R. M.
Right arrow Articles by Galbraith, D. W.

Received February 28, 2007
Accepted May 5, 2007

High-Throughput, High-Sensitivity Analysis of Gene Expression in Arabidopsis

Richard Martin Kris *, Stephen Felder , Michael Deyholos , Georgina M. Lambert , James Hinton , Ihab Botros , Ralph Martel , Bruce Seligmann , and David W. Galbraith

NuvoGen Research, L.L.C., P.O. Box 64326, Tucson, Arizona 85728; High Throughput Genomics, Inc., 6296 E. Grant Road, Tucson, Arizona 85712; Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721

* Corresponding author; email: rkris{at}nuvogenresearch.com.

High-throughput gene expression analysis of genes expressed during salt stress was performed using a novel multiplexed quantitative Nuclease Protection Assay that involves customized DNA microarrays printed within the individual wells of 96-well plates. The levels of expression of the transcripts from sixteen different genes were quantified within crude homogenates prepared from Arabidopsis thaliana plants also grown in a 96-well plate format. Examples are provided of the high degree of reproducibility of quantitative dose-response data, and of the sensitivity of detection of changes in gene expression within limiting amounts of tissue. The lack of requirement for RNA purification renders the assay particularly suited for high-throughput gene expression analysis, and for the discovery of novel chemical compounds that specifically modulate the expression of endogenous target genes.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Arany, B. K. Wagner, Y. Ma, J. Chinsomboon, D. Laznik, and B. M. Spiegelman
Gene expression-based screening identifies microtubule inhibitors as inducers of PGC-1{alpha} and oxidative phosphorylation
PNAS, March 25, 2008; 105(12): 4721 - 4726.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists