Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on May 18, 2007; 10.1104/pp.107.098731

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrowOA All Versions of this Article:
144/3/1336    most recent
pp.107.098731v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sakurai, I.
Right arrow Articles by Wada, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sakurai, I.
Right arrow Articles by Wada, H.
Agricola
Right arrow Articles by Sakurai, I.
Right arrow Articles by Wada, H.

Received March 1, 2007
Accepted May 14, 2007

Effects of the Lack of Phosphatidylglycerol on the Donor Side of Photosystem II

Isamu Sakurai , Naoki Mizusawa , Shunsuke Ohashi , Masami Kobayashi , and Hajime Wada *

Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo 153-8902, Japan; Institute of Materials Science, University of Tsukuba, Ibaraki 305-8573, Japan

* Corresponding author; email: hwada{at}bio.c.u-tokyo.ac.jp.

Our previous studies with the pgsA mutant of the cyanobacterium Synechocystis sp. PCC6803 (hereafter termed pgsA mutant), which is defective for the biosynthesis of phosphatidylglycerol (PG), revealed an important role for PG in the electron acceptor side of photosystem II (PSII), especially in the electron transport between plastoquinones QA and QB. The present study now shows that PG also plays an important role in the electron donor side of PSII, namely the oxygen-evolving system. Analyses of purified PSII complexes indicated that PSII from PG-depleted pgsA mutant cells sustained only ~50% of the oxygen-evolving activity compared to wild-type cells. Dissociation of the extrinsic proteins PsbO, PsbV, and PsbU, which are required for stabilization of the Mn-cluster, followed by the release of a manganese atom, was observed in PSII of the PG-depleted mutant cells. The released PsbO re-bound to PSII when PG was added back to the PG-depleted mutant cells, even when de novo protein synthesis was inhibited. Changes in photosynthetic activity of the PG-depleted pgsA mutant cells induced by heat treatment or dark incubation resembled those of {Delta}psbO, {Delta}psbV and {Delta}psbU mutant cells. These results suggest that PG plays an important role in the binding of extrinsic proteins required for sustaining a functional Mn-cluster on the donor side of PSII.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
M. Watanabe, M. Iwai, R. Narikawa, and M. Ikeuchi
Is the Photosystem II Complex a Monomer or a Dimer?
Plant Cell Physiol., September 1, 2009; 50(9): 1674 - 1680.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Takahashi, S. M. Whitney, and M. R. Badger
Different thermal sensitivity of the repair of photodamaged photosynthetic machinery in cultured Symbiodinium species
PNAS, March 3, 2009; 106(9): 3237 - 3242.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Dobakova, R. Sobotka, M. Tichy, and J. Komenda
Psb28 Protein Is Involved in the Biogenesis of the Photosystem II Inner Antenna CP47 (PsbB) in the Cyanobacterium Synechocystis sp. PCC 6803
Plant Physiology, February 1, 2009; 149(2): 1076 - 1086.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. Sakurai, N. Mizusawa, H. Wada, and N. Sato
Digalactosyldiacylglycerol Is Required for Stabilization of the Oxygen-Evolving Complex in Photosystem II
Plant Physiology, December 1, 2007; 145(4): 1361 - 1370.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists