Plant Physiology Preview
Published on May 11, 2007; 10.1104/pp.107.099317
OPEN ACCESS ARTICLE
Received March 10, 2007
Accepted May 6, 2007
Deletion of Glycine Decarboxylase in Arabidopsis Is Lethal under Non-Photorespiratory Conditions
Nadja Engel , Kirsten van den Daele , Üner Kolukisaoglu , Katja Morgenthal , Wolfram Weckwerth , Tiit Pärnik , Olav Keerberg , and Hermann Bauwe *
Department of Plant Physiology, University of Rostock, Albert-Einstein-Straße 3, D-18059 Rostock, Germany; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Golm, Germany,; Estonian University of Life Sciences, Institute of Agricultural and Environmental Sciences, 51014 Tartu, Estonia
* Corresponding author; email: hermann.bauwe{at}uni-rostock.de.
The mitochondrial multienzyme Gly decarboxylase (GDC) catalyzes the tetrahydrofolate (THF)-dependent catabolism of Gly to 5,10-methylene-THF and the side-products NADH, CO2, and NH3. This reaction forms part of the photorespiratory cycle and contributes to one-carbon metabolism. While the important role of GDC for these two metabolic pathways is well established, the existence of bypassing reactions has also been suggested. Therefore, it is not clear to what extent GDC is obligatory for these processes. Here, we report on features of individual and combined T-DNA insertion mutants for one of the GDC subunits, P-protein, which is encoded by two genes in Arabidopsis thaliana. The individual knockout of either of these two genes does not significantly alter metabolism and photosynthetic performance indicating functional redundancy. In contrast, the double-mutant does not develop beyond the cotyledon stage in air enriched with 0.9% CO2. Rosette leaves do not appear and the seedlings do not survive for longer than about three to four weeks under these non-photorespiratory conditions. This feature distinguishes the GDC-lacking double-mutant from all other known photorespiratory mutants and provides evidence for the non-replaceable function of GDC in vital metabolic processes other than photorespiration.
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