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Published on April 27, 2007; 10.1104/pp.107.099473

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Received March 13, 2007
Accepted April 17, 2007

Down-regulation of a Silent Information Regulator2 -Related Gene, OsSRT1 Induces DNA Fragmentation and Cell Death in Rice

Limin Huang , Qianwen Sun , Fujun Qin , Chen Li , Yu Zhao , and Dao-Xiu Zhou *

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070 China; Department of Quartermaster, Military Economy Academy, Wuhan, 430035 China; Institut de biotechnologie des Plantes, Université Paris sud 11, 91405 Orsay, France

* Corresponding author; email: dao-xiu.zhou{at}u-psud.fr.

The Silent information regulator2 (SIR2) family proteins are NAD+-dependent histone deacetylases. Sir2 is involved in chromatin silencing at the mating type loci, rDNA and telomeres in yeast and is associated with lifespan extension in yeast, worms and flies, but also in a broader range of additional functions. In this work, we investigated the role of OsSRT1, one of the two SIR2-related genes found in rice (Oryza sativa). We show that OsSRT1 was a widely expressed nuclear protein with higher levels in rapidly dividing tissues. OsSRT1 RNAi induced an increase of histone H3K9 acetylation and a decrease of H3K9 dimethylation, leading to H2O2 production, DNA fragmentation, cell death and lesions mimicking plant hypersensitive responses during incompatible interactions with pathogens, whereas over-expression of OsSRT1 enhanced tolerance to oxidative stress. Transcript microarray analysis revealed that the transcription of many transposons and retrotransposons in addition to genes related to hypersensitive response and/or programmed cell death was activated. Chromatin immunorepcipitation assays showed that OsSRT1 down-regulation induced histone H3K9 acetylation on the transposable elements and some of the HR-related genes, suggesting that these genes may be amongst the primary targets of deacetylation regulated by OsSRT1. Our data together suggest that the rice SIR2-like gene is required for safeguard against genome instability and cell damage to ensure plant cell growth, but likely implicating different molecular mechanisms than yeast and animal homologues.




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