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Published on May 25, 2007; 10.1104/pp.107.099762

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Received March 19, 2007
Accepted April 29, 2007

Ethylene Insensitivity Results in a Down-Regulation of Rubisco Expression and Photosynthetic Capacity in Nicotiana tabacum

Danny Tholen , Thijs L. Pons , Laurentius A.C.J. Voesenek , and Hendrik Poorter *

Plant Ecophysiology, Institute of Environmental Biology, Utrecht University, P.O. Box 800.84, 3508 TB Utrecht, The Netherlands

* Corresponding author; email: H.Poorter{at}uu.nl.

Little is known about the effect of hormones on the photosynthetic process. Therefore, we studied Rubisco content and expression along with gas exchange parameters in transgenic tobacco (Nicotiana tabacum) plants that are not able to sense ethylene. We also tested for a possible interaction between ethylene-insensitivity, ABA and sugar feedback on photosynthesis. We measured Rubisco content in seedlings grown in agar with or without added sugar and fluridone, and Rubisco expression in hydroponically-grown vegetative plants grown at low and high CO2. Furthermore, we analyzed gas exchange and the photosynthetic machinery of transformants and wild type (WT) plants grown under standard conditions. In the presence of exogenous glucose, agar-grown seedlings of the ethylene-insensitive genotype had lower amounts of Rubisco per unit leaf area than the wild type. No differences in Rubisco content were found between ethylene-insensitive and wild type seedlings treated with fluridone, suggesting that inhibition of ABA production nullified the effect of glucose application. When larger, vegetative plants were grown at different atmospheric CO2 concentrations, a negative correlation was found between glucose concentration in the leaves and Rubisco gene expression, with a stronger repression by high glucose concentrations in the ethylene-insensitive plants. Ethylene insensitivity resulted in plants with comparable fractions of N invested in light harvesting, but lower amounts in electron transport and Rubisco. Consequently, photosynthetic capacity of the insensitive genotype was clearly lower compared to the wild type. We conclude that the inability to perceive ethylene results in an increased sensitivity to glucose, which may be mediated by a higher ABA concentration. This increased sensitivity to endogenous glucose has negative consequences for the Rubisco content and the photosynthetic capacity of these plants.




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A. Boonman, E. Prinsen, L. A. C. J. Voesenek, and T. L. Pons
Redundant roles of photoreceptors and cytokinins in regulating photosynthetic acclimation to canopy density
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[Abstract] [Full Text] [PDF]


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