Plant Physiology Preview
Published on April 27, 2007; 10.1104/pp.107.100172
OPEN ACCESS ARTICLE
Received March 25, 2007
Accepted April 13, 2007
Identification of a Novel Chloroplast Protein AtNYE1 Regulating Chlorophyll Degradation during Leaf Senescence in Arabidopsis
Guodong Ren , Kun An , Yang Liao , Xiao Zhou , Yajun Cao , Huifang Zhao , Xiaochun Ge , and Benke Kuai *
State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200433, China
* Corresponding author; email: bbkuai{at}fudan.edu.cn.
A dramatic increase of chlorophyll (Chl) degradation occurs during senescence of vegetative plant organs and fruit ripening. Although the biochemical pathway of Chl degradation has long been proposed, little is known about its regulatory mechanism. Identification of Chl degradation-disturbed mutants and subsequently isolation of responsible genes would greatly facilitate the elucidation of the regulation of Chl degradation. Here, we describe a non-yellowing mutant of Arabidopsis, nye1-1, in which 50% Chls was retained, compared to less than 10% in the wild type (Col-0), at the end of a six-day dark incubation. Nevertheless, neither photosynthesis- nor senescence-associated process was significantly affected in nye1-1. Characteristically, a significant reduction in PaO activity was detected in nye1-1. However, no detectable accumulation of either chlorophyllide a or pheophorbide a was observed. Reciprocal crossings revealed that the mutant phenotype was caused by a monogenic semi-dominant nuclear mutation. We have identified AtNYE1 by positional cloning. Dozens of its putative orthologs, predominantly appeared in higher plant species, were identified, some of which have been associated with Chl degradation in a few crop species. qPCR analysis showed that AtNYE1 was drastically induced by senescence signals. Constitutive over-expression of AtNYE1 could result in either pale-yellow true leaves or even albino seedlings. These results collectively indicate that NYE1 plays an important regulatory role in Chl degradation during senescence by modulating PaO activity.
This article has been cited by other articles:
|
|
|
|
 
X. Yang, X. Pang, L. Xu, R. Fang, X. Huang, P. Guan, W. Lu, and Z. Zhang
Accumulation of soluble sugars in peel at high temperature leads to stay-green ripe banana fruit
J. Exp. Bot.,
October 1, 2009;
60(14):
4051 - 4062.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Schelbert, S. Aubry, B. Burla, B. Agne, F. Kessler, K. Krupinska, and S. Hortensteiner
Pheophytin Pheophorbide Hydrolase (Pheophytinase) Is Involved in Chlorophyll Breakdown during Leaf Senescence in Arabidopsis
PLANT CELL,
March 1, 2009;
21(3):
767 - 785.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Wang, T. Lai, G. Qin, and S. Tian
Response of Jujube Fruits to Exogenous Oxalic Acid Treatment Based on Proteomic Analysis
Plant Cell Physiol.,
February 1, 2009;
50(2):
230 - 242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Alos, M. Roca, D. J. Iglesias, M. I. Minguez-Mosquera, C. M. B. Damasceno, T. W. Thannhauser, J. K. C. Rose, M. Talon, and M. Cercos
An Evaluation of the Basis and Consequences of a Stay-Green Mutation in the navel negra Citrus Mutant Using Transcriptomic and Proteomic Profiling and Metabolite Analysis
Plant Physiology,
July 1, 2008;
147(3):
1300 - 1315.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. Barry, R. P. McQuinn, M.-Y. Chung, A. Besuden, and J. J. Giovannoni
Amino Acid Substitutions in Homologs of the STAY-GREEN Protein Are Responsible for the green-flesh and chlorophyll retainer Mutations of Tomato and Pepper
Plant Physiology,
May 1, 2008;
147(1):
179 - 187.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Phillips, A. Suttangkakul, and R. D. Vierstra
The ATG12-Conjugating Enzyme ATG10 Is Essential for Autophagic Vesicle Formation in Arabidopsis thaliana
Genetics,
March 1, 2008;
178(3):
1339 - 1353.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Sato, R. Morita, M. Nishimura, H. Yamaguchi, and M. Kusaba
Mendel's green cotyledon gene encodes a positive regulator of the chlorophyll-degrading pathway
PNAS,
August 28, 2007;
104(35):
14169 - 14174.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
