Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on May 25, 2007; 10.1104/pp.107.100321

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
145/1/29    most recent
pp.107.100321v2
pp.107.100321v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, Z.
Right arrow Articles by Wan, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, Z.
Right arrow Articles by Wan, J.
Agricola
Right arrow Articles by Wu, Z.
Right arrow Articles by Wan, J.

Received April 1, 2007
Accepted May 16, 2007

A Chlorophyll-Deficient Rice Mutant with Impaired Chlorophyllide Esterification in Chlorophyll Biosynthesis

Ziming Wu , Xin Zhang , Bin He , Liping Diao , Shenglan Sheng , Jiulin Wang , Xiuping Guo , Ning Su , Lifeng Wang , Ling Jiang , Chunming Wang , Huqu Zhai , and Jianmin Wan *

National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China; The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing100081, China; Zhenjiang Agriculture Science Institute, Zhenjiang 212400, China

* Corresponding author; email: wanjm{at}caas.net.cn.

Chlorophyll synthase catalyzes esterification of chlorophyllide to complete the last step of chlorophyll biosynthesis. Although the chlorophyll synthases and the corresponding genes from various organisms have been well characterized, chlorophyll synthase mutants have not yet been reported in higher plants. In this study, a rice (Oryza Sativa L.) chlorophyll-deficient mutant, yellow-green leaf 1 (ygl1), was isolated, which showed yellow-green leaves in young plants with decreased chlorophyll synthesis, increased level of tetrapyrrole intermediates, and delayed chloroplast development. Genetic analysis demonstrated that the phenotype of ygl1 was caused by a recessive mutation in a nuclear gene. The ygl1 locus was mapped to chromosome 5 and isolated by map-based cloning. Sequence analysis revealed that it encodes the chlorophyll synthase and its identity was verified by transgenic complementation. A missense mutation was found in a highly conserved residue of YGL1 in the ygl1 mutant, resulting in reduction of the enzymatic activity. YGL1 is constitutively expressed in all tissues, and its expression is not significantly affected in the ygl1 mutant. Interestingly, the mRNA expression of the cab1R gene encoding the Chl a/b-binding protein was severely suppressed in the ygl1 mutant. Moreover, the expression of some nuclear genes associated with chlorophyll biosynthesis or chloroplast development was also affected in ygl1 seedlings. These results indicate that the expression of nuclear genes encoding various chloroplast proteins might be feedback regulated by the level of chlorophyll or chlorophyll precursors.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
W. Y. Bang, I. S. Jeong, D. W. Kim, C. H. Im, C. Ji, S. M. Hwang, S. W. Kim, Y. S. Son, J. Jeong, T. Shiina, et al.
Role of Arabidopsis CHL27 Protein for Photosynthesis, Chloroplast Development and Gene Expression Profiling
Plant Cell Physiol., September 1, 2008; 49(9): 1350 - 1363.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists