Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on April 20, 2007; 10.1104/pp.107.100404

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
144/2/976    most recent
pp.107.100404v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Woodward, A. W.
Right arrow Articles by Bartel, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Woodward, A. W.
Right arrow Articles by Bartel, B.
Agricola
Right arrow Articles by Woodward, A. W.
Right arrow Articles by Bartel, B.

Received April 2, 2007
Accepted April 4, 2007

Mutation of E1-CONJUGATING ENZYME-RELATED1 Decreases RUB Conjugation and Alters Auxin Response and Development

Andrew W. Woodward , Sarah E. Ratzel , Erin E. Woodward , Yousif Shamoo , and Bonnie Bartel *

Department of Biochemistry and Cell Biology, 6100 Main St., Rice University, Houston, TX 77005

* Corresponding author; email: bartel{at}rice.edu.

The ubiquitin-like protein RUB is conjugated to CULLIN proteins to modulate the activity of SCF ubiquitylation complexes. RUB conjugation to specific target proteins is necessary for the development of many organisms, including Arabidopsis thaliana. Here, we report the isolation and characterization of ecr1-1, an Arabidopsis mutant compromised in RUB activation. The ecr1-1 mutation causes a missense change located two amino acid residues from the catalytic site cysteine that normally functions to form a thioester bond with activated RUB. A higher ratio of unmodified CULLIN1 (CUL1) relative to CUL1-RUB is present in ecr1-1 compared to wild type, suggesting that the mutation reduces ECR1 function. The ecr1-1 mutant is resistant to the auxin-like compound indole-3-propionic acid, produces fewer lateral roots than wild type, displays reduced adult height, and stabilizes a reporter fusion protein that is degraded in response to auxin, suggesting reduced auxin signaling in the mutant. In addition, ecr1-1 hypocotyls fail to elongate normally when seedlings are grown in darkness, a phenotype shared with certain other RUB conjugation mutants that is not general to auxin-response mutants. The suite of ecr1-1 molecular and morphological phenotypes reflects roles for RUB conjugation in many aspects of plant growth and development. Certain ecr1-1 elongation defects are restored by treatment with the ethylene response inhibitor silver nitrate, suggesting that the short ecr1-1 root and hypocotyl result from aberrant ethylene accumulation. Further, silver nitrate supplementation in combination with various auxins and auxin-like compounds reveals that members of this growth regulator family may differentially rely on ethylene signaling to inhibit root growth.




This article has been cited by other articles:


Home page
 GeneticsHome page
J. Gilkerson, J. Hu, J. Brown, A. Jones, T.-p. Sun, and J. Callis
Isolation and Characterization of cul1-7, a Recessive Allele of CULLIN1 That Disrupts SCF Function at the C Terminus of CUL1 in Arabidopsis thaliana
Genetics, March 1, 2009; 181(3): 945 - 963.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. K. Biswas, C. Ooura, K. Higuchi, Y. Miyazaki, V. Van Nguyen, A. Rahman, H. Uchimiya, T. Kiyosue, T. Koshiba, A. Tanaka, et al.
Genetic Characterization of Mutants Resistant to the Antiauxin p-Chlorophenoxyisobutyric Acid Reveals That AAR3, a Gene Encoding a DCN1-Like Protein, Regulates Responses to the Synthetic Auxin 2,4-Dichlorophenoxyacetic Acid in Arabidopsis Roots
Plant Physiology, November 1, 2007; 145(3): 773 - 785.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists