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Published on May 11, 2007; 10.1104/pp.107.100842

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Received April 10, 2007
Accepted May 7, 2007

Phosphatidylinositol 4-kinase Activation Is an Early Response to Salicylic Acid in Arabidopsis Suspension Cells

Ondrej Krinke *, Eric Ruelland , Olga Valentová , Chantal Vergnolle , Jean-Pierre Renou , Ludivine Taconnat , Matyás Flemr , Lenka Burketová , and Alain Zachowski

Université Pierre et Marie Curie-Paris 6, Unité Mixte de Recherche 7180, Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes, Ivry-sur-Seine, F-94200 France; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7180, Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes, Ivry-sur-Seine, F-94200 France; Institute of Chemical Technology, Prague, Department of Biochemistry and Microbiology, Prague, 166 28 Czech Republic; Unité Mixte de Recherche Institut National de la Recherche Agronomique 1165; Centre National de la Recherche Scientifique 8114, Unité de Recherche en Génomique Végétale, Evry, F-91057 France; The Academy of Sciences of the Czech Republic, Institute of Experimental Botany, v.v.i., Prague, 160 00 Czech Republic

* Corresponding author; email: ondrej.krinke{at}vscht.cz.

Salicylic acid (SA) has a central role in defence against pathogen attack. Besides, its role in such diverse processes as germination, flowering, senescence and thermotolerance acquisition has been documented. However, little is known about the early signalling events triggered by SA. Using Arabidopsis thaliana suspension cells as a model, it was possible to show by in vivo metabolic phospholipid labelling with 33Pi that SA addition induced a rapid and early (in few minutes) decrease in a pool of phosphatidylinositol (PI). This decrease paralleled with an increase in phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate. These changes could be inhibited by two different inhibitors of type III PI 4-kinases, phenylarsine oxide and 30 µM wortmannin; no inhibitory effect was seen with 1 µM wortmannin, a concentration inhibiting PI 3-kinases but not PI 4-kinases. We therefore undertook a study of wortmannin effects on SA responsive transcriptome. Using the Complete Arabidopsis Transcriptome MicroArray (CATMA) chip, we could identify 774 genes differentially expressed upon SA treatment. Strikingly, amongst these genes, the response to SA of 112 of them was inhibited by 30 µM wortmannin but not by 1 µM wortmannin.






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