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Published on June 7, 2007; 10.1104/pp.107.100867

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Received April 13, 2007
Accepted June 3, 2007

How a Plant Lectin Recognizes High Mannose oligosaccharides

Abel Garcia-Pino , Lieven Buts , Lode Wyns , Anne Imberty , and Remy Loris *

Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium; Department of Molecular and Cellular Interactions, VIB, Pleinlaan 2, B-1050 Brussel, Belgium; Centre de Recherches sur les Macromolécules Végétales, CNRS (affiliated with Joseph Fourier), BP53, 38041 Grenoble cedex 09, France

* Corresponding author; email: reloris{at}vub.ac.be.

The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine mannose residues of Man-9 have the potential to bind in the monosaccharide binding site, all oligomannoses are bound in the same unique way employing the tetrasaccharide sequence Man{alpha}(1-2)Man{alpha}(1-6)[Man{alpha}(1-3)]Man{alpha}(1-. ITC titration experiments using Man-5, Man-9 and the Man-9-containing glycoprotein soybean agglutinin as ligands confirm the monovalence of Man-9 and show a four times higher affinity for Man-9 when it is presented to Pterocarpus angolensis seed lectin in a glycoprotein context.






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