Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on June 7, 2007; 10.1104/pp.107.101600

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
144/4/1797    most recent
pp.107.101600v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, M.
Right arrow Articles by Xue, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, M.
Right arrow Articles by Xue, Y.
Agricola
Right arrow Articles by Li, M.
Right arrow Articles by Xue, Y.

Received April 26, 2007
Accepted May 24, 2007

Genome-Wide Gene Expression Profiling Reveals Conserved and Novel Molecular Functions of the Stigma in Rice (Oryza sativa L.)

Meina Li , Wenying Xu , Wenqiang Yang , Zhaosheng Kong , and Yongbiao Xue *

Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, Beijing 100080, China; Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

* Corresponding author; email: ybxue{at}genetics.ac.cn.

In angiosperms, stigma provides initial nutrients and guidance cues for pollen grain germination and tube growth. However, little is known about the genes that regulate these processes in rice. Here we generate rice stigma-specific or -preferential gene expression profiles through comparing genome-wide expression patterns of hand dissected unpollinated stigma at anthesis with seven tissues including seedling shoot, seedling root, mature anther, ovary at anthesis, seeds of five days after pollination, 10-day-old embryo, 10-day-old endosperm as well as suspension cultured cells by using both 57K Affymetrix rice whole genome array and 10K rice cDNA microarray. A high reproducibility of the microarray results was detected between the two different technology platforms. In total, we identified 548 genes to be expressed specifically or predominantly in the stigma papillar cells of rice. Real-Time quantitative RT-PCR analysis of 34 selected genes all confirmed their stigma-specific expression. The expression of five selected genes was further validated by RNA in situ hybridization. Gene Ontology (GO) analysis shows that several auxin-signaling components, transcription and stress-related genes are significantly overrepresented in the rice stigma gene set. Interestingly, most of them also share several cis-regulatory elements with known stress-responsive genes, supporting the notion of an overlap of genetic programs regulating pollination and stress/defense responses. We also found that genes involved in cell wall metabolism and cellular communication appear to be conserved in the stigma between rice and Arabidopsis. Our results indicate that the stigmas appear to have conserved and novel molecular functions between rice and Arabidopsis.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. C. Quiapim, M. S. Brito, L. A.S. Bernardes, I. daSilva, I. Malavazi, H. C. DePaoli, J. B. Molfetta-Machado, S. Giuliatti, G. H. Goldman, and M. H. S. Goldman
Analysis of the Nicotiana tabacum Stigma/Style Transcriptome Reveals Gene Expression Differences between Wet and Dry Stigma Species
Plant Physiology, March 1, 2009; 149(3): 1211 - 1230.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Huang, N. L. Taylor, R. Narsai, H. Eubel, J. Whelan, and A. H. Millar
Experimental Analysis of the Rice Mitochondrial Proteome, Its Biogenesis, and Heterogeneity
Plant Physiology, February 1, 2009; 149(2): 719 - 734.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. A. Howell, R. Narsai, A. Carroll, A. Ivanova, M. Lohse, B. Usadel, A. H. Millar, and J. Whelan
Mapping Metabolic and Transcript Temporal Switches during Germination in Rice Highlights Specific Transcription Factors and the Role of RNA Instability in the Germination Process
Plant Physiology, February 1, 2009; 149(2): 961 - 980.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
W.-Q. Yang, Y. Lai, M.-N. Li, W.-Y. Xu, and Y.-B. Xue
A Novel C2-Domain Phospholipid-Binding Protein, OsPBP1, Is Required for Pollen Fertility in Rice
Mol Plant, September 1, 2008; 1(5): 770 - 785.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists