Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Published on June 1, 2007; 10.1104/pp.107.101618

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Received April 26, 2007
Accepted May 21, 2007

Medicago truncatula Root Nodule Proteome Analysis Reveals Differential Plant and Bacteroid Responses to Drought Stress

Estíbaliz Larrainzar , Stefanie Wienkoop , Wolfram Weckwerth , Rubén Ladrera , Cesar Arrese-Igor , and Esther M. González *

Dpto. Ciencias del Medio Natural, Universidad Pública de Navarra, 31006 Pamplona, Navarra, Spain; Proteome Factory, Dorotheenstr. 94, 10117 Berlin, Germany; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, Germany

* Corresponding author; email: esther.gonzalez{at}unavarra.es.

Drought is one of the environmental factors most affecting crop production. Under drought symbiotic nitrogen fixation is one of the physiological processes to first show stress responses in nodulated legumes. This inhibition process involves a number of factors whose interactions are not yet understood. The present work aims to further understand changes occurring in nodules under drought stress from a proteomic perspective. Drought was imposed on Medicago truncatula cv. Jemalong A17 plants grown in symbiosis with Sinorhizobium meliloti 2011. Changes at the protein level were analysed using a non-gel approach based on liquid chromatography coupled to tandem mass spectrometry. Due to the complexity of nodule tissue, the separation of plant and bacteroid fractions in M. truncatula root nodules was first checked with the aim of minimising cross-contamination between the fractions. Secondly, the protein plant fraction of M. truncatula nodules was profiled, leading to the identification of 377 plant proteins, the largest description of the plant nodule proteome so far. Thirdly, both symbiotic partners were independently analysed for quantitative differences at the protein level during drought stress. Multivariate data mining allowed for the classification of proteins sets which were involved in drought stress responses. The isolation of the nodule plant and bacteroid protein fractions enabled the independent analysis of the response of both counterparts, gaining further understanding of how each symbiotic member is distinctly affected at the protein level under a water-deficit situation.




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