Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on July 27, 2007; 10.1104/pp.107.101741

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrowOA All Versions of this Article:
145/1/236    most recent
pp.107.101741v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, S.
Right arrow Articles by Franklin-Tong, V. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, S.
Right arrow Articles by Franklin-Tong, V. E.
Agricola
Right arrow Articles by Li, S.
Right arrow Articles by Franklin-Tong, V. E.

Received May 9, 2007
Accepted July 17, 2007

A MAPK Signals to Programmed Cell Death Induced by Self-Incompatibility in Papaver Pollen

Shutian Li , Jozef Samaj , and Vernonica E. Franklin-Tong *

School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, U.K; Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115 Bonn, Germany; Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka 2, 950 07 Nitra, Slovak Republic

* Corresponding author; email: V.E.Franklin-Tong{at}bham.ac.uk.

Self-incompatibility (SI) in higher plants is an important mechanism to prevent inbreeding and involves specific rejection of incompatible ("self") pollen. In Papaver rhoeas, S proteins encoded by the stigma component of the S-locus interact with incompatible pollen, resulting in cessation of tip growth. This "self" interaction triggers a Ca2+-dependent signaling network, involving programmed cell death (PCD).

We previously identified p56, a mitogen activated protein kinase (MAPK) that is activated during the SI response in incompatible pollen. Here we show that p56 crossreacts with AtMPK3, but not with AtMPK4 or SIPK antibodies. We provide good evidence that a MAPK is involved in initiation of SI-induced PCD in incompatible pollen. SI rapidly reduces pollen viability and the MAPK cascade inhibitor U0126, which prevents the SI-induced activation of the p56 in incompatible pollen, "rescues" incompatible pollen, while its negative analog, U0124, does not. This strongly implicates the involvement of a MAPK in SI-mediated loss of pollen viability and cell death. SI also stimulates a caspase-3-like (DEVDase) activity and later DNA fragmentation. Both these markers of PCD are significantly reduced by pretreatment with U0126, implicating the involvement of a MAPK in signaling during early PCD. As p56 appears to be the only MAPK activated by SI, our studies imply that p56 could be the MAPK involved in mediating SI-induced PCD.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. Pitzschke and H. Hirt
Disentangling the Complexity of Mitogen-Activated Protein Kinases and Reactive Oxygen Species Signaling
Plant Physiology, February 1, 2009; 149(2): 606 - 615.
[Full Text] [PDF]

Home page
 Mol PlantHome page
M. Bosch, N. S. Poulter, S. Vatovec, and V. E. Franklin-Tong
Initiation of Programmed Cell Death in Self-Incompatibility: Role for Cytoskeleton Modifications and Several Caspase-Like Activities
Mol Plant, November 1, 2008; 1(6): 879 - 887.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. B. Lee, K. N. Swatek, and B. McClure
Pollen Proteins Bind to the C-terminal Domain of Nicotiana alata Pistil Arabinogalactan Proteins
J. Biol. Chem., October 3, 2008; 283(40): 26965 - 26973.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. S. Poulter, S. Vatovec, and V. E. Franklin-Tong
Microtubules Are a Target for Self-Incompatibility Signaling in Papaver Pollen
Plant Physiology, March 1, 2008; 146(3): 1358 - 1367.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Bosch and V. E. Franklin-Tong
Temporal and spatial activation of caspase-like enzymes induced by self-incompatibility in Papaver pollen
PNAS, November 13, 2007; 104(46): 18327 - 18332.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists