Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Published on September 7, 2007; 10.1104/pp.107.102236

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Received May 10, 2007
Accepted August 31, 2007

Rice P1B-type Heavy Metal ATPase, OsHMA9, Is a Metal Efflux Protein

Sichul Lee , Yu-Young Kim , Youngsook Lee , and Gynheung An *

Division of Molecular and Life Science, and Biotechnology Research Center, Pohang University of Science and Technology, Pohang 790-784, Korea

* Corresponding author; email: genean{at}postech.ac.kr.

P1B-type heavy metal ATPases are transmembrane metal-transporting proteins that play a key role in metal homeostasis. Despite their importance, very little is known about their functions in monocot species. We report the characterization of rice (Oryza sativa) OsHMA9, a member of the P1B-type ATPase family. Semi-quantitative RT-PCR analyses of seedlings showed that OsHMA9 expression was induced by a high concentration of copper, zinc and cadmium. We also determined, through promoter::{beta}-glucuronidase analysis, that the main expression was in the vascular bundles and anthers. The OsHMA9: green fluorescence protein fusion was localized to the plasma membrane. Heterologous expression of OsHMA9 partially rescued the copper sensitivity of the Escherichia coli copA mutant which is defective in Cu-transporting ATPases. It did not rescue the Zn-sensitivity of the zntA mutant, which is defective in Zn-transporting ATPase. To further elucidate the functional roles of OsHMA9, we isolated two independent null alleles, oshma9-1 and oshma9-2, from the T-DNA insertion population. Mutant plants exhibited the phenotype of increased sensitivity to elevated levels of Cu, Zn, and Pb. These results support a role for OsHMA9 in Cu, Zn and Pb efflux from the cells. This paper is the first report on the functional characterization of a P1B-type metal efflux transporter in monocots.




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