Plant Physiol. Bio-Rad Microplate Reader
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on July 20, 2007; 10.1104/pp.107.102285

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrowOA All Versions of this Article:
145/1/119    most recent
pp.107.102285v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saracco, S. A.
Right arrow Articles by Vierstra, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saracco, S. A.
Right arrow Articles by Vierstra, R. D.
Agricola
Right arrow Articles by Saracco, S. A.
Right arrow Articles by Vierstra, R. D.

Received May 11, 2007
Accepted July 5, 2007

Genetic Analysis of Sumoylation in Arabidopsis: Heat-induced Conjugation of SUMO1 AND 2 Is Essential

Scott A. Saracco , Marcus J. Miller , Jasmina Kurepa , and Richard D. Vierstra *

Department of Genetics, 425-G Henry Mall, University of Wisconsin-Madison, Madison, WI 53706-1574

* Corresponding author; email: vierstra{at}wisc.edu.

The post-translational addition of Small-Ubiquitin-Like Modifiers (SUMOs) to other intracellular proteins has been implicated in a variety of eukaryotic functions, including modifying cytoplasmic signal transduction, nuclear import and subnuclear compartmentalization, DNA repair, and transcription regulation. For plants in particular, both genetic analyses and the rapid accumulation of SUMO conjugates in response to various adverse environmental conditions suggest that SUMOylation plays a key role in the stress response. Through genetic analyses of various SUMO conjugation mutants, we show here that the SUMO1 and 2 isoforms in particular and SUMOylation in general are essential for viability in Arabidopsis thaliana. Null T-DNA insertion mutants affecting the single genes encoding the SUMO-activating enzyme subunit SAE2 and the SUMO-conjugating enzyme SCE1 are embryonic lethal, with the arrest occurring early in embryo development. Whereas the single genes encoding the SUMO1 and SUMO2 isoforms are not essential by themselves, double mutants missing both are also embryonic lethal. Viability can be restored by reintroduction of SUMO1 expression in the homozygous sum1-1 sum2-1 background. Various stresses like heat shock dramatically increase the pool of SUMO conjugates in planta. This increase involves SUMO1 and 2 and is mainly driven by the SUMO-protein ligase SIZ1, with most of the conjugates accumulating in the nucleus. Taken together, it appears that SIZ1-mediated conjugation of SUMO1 and 2 to other intracellular proteins is essential in Arabidopsis, possibly through stress-induced modification of a potentially diverse pool of nuclear proteins.




This article has been cited by other articles:


Home page
 J Exp BotHome page
Y. Lee, H.-S. Lee, J.-S. Lee, S.-K. Kim, and S.-H. Kim
Hormone- and light-regulated nucleocytoplasmic transport in plants: current status
J. Exp. Bot., September 1, 2008; 59(12): 3229 - 3245.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Garcia-Dominguez, R. March-Diaz, and J. C. Reyes
The PHD Domain of Plant PIAS Proteins Mediates Sumoylation of Bromodomain GTE Proteins
J. Biol. Chem., August 1, 2008; 283(31): 21469 - 21477.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists