Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Published on September 20, 2007; 10.1104/pp.107.102657

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Received May 22, 2007
Accepted September 10, 2007

A Non-Classical Arabinogalactan-Protein Highly Expressed in Vascular Tissues, AGP31, Is Transcriptionally Repressed by Methyl Jasmonic Acid in Arabidopsis

Chenggang Liu and Mona C. Mehdy *

Section of Molecular, Cell and Developmental Biology, The University of Texas at Austin, Austin, TX 78712

* Corresponding author; email: mmehdy{at}mail.utexas.edu.

In response to wounding and pathogens, jasmonate serves as a signal molecule for both induction and repression of gene expression. To examine defense-regulated gene repression in Arabidopsis (Arabidopsis thaliana), we have identified a non-classical arabinogalactan-protein (AGP) gene, AGP31, and show that its mRNA decreased to about 30% of its original level within 8 hr in response to methyl jasmonate (MeJA) treatment of whole 7-day-old seedlings. Wounding and abscisic acid (ABA) treatments had similar effects. MeJA suppression primarily depends on the action of the JA-signaling protein, COI1, as shown by much lower MeJA suppression in coi1-1 mutant plants. The main mechanism of mRNA suppression by MeJA is repression of transcription, as shown by nuclear run-on experiments. The AGP31 protein shares features with several known and putative non-classical AGPs from other species: a putative signal peptide, a His-rich region near the N-terminus followed by a repetitive proline-rich domain and a cysteine-rich C-terminal PAC domain. Positive Yariv reagent interaction demonstrated that the protein is an AGP. Monosaccharide analysis of purified AGP31 indicated it is a galactose-rich AGP. Expression of an AGP31-eGFP fusion protein in transgenic cells revealed that the AGP31 protein was localized to the cell wall. AGP31 promoter-GUS reporter gene analysis showed expression in the vascular bundle throughout the plant except in the flower. In the flower, GUS staining occurred throughout the pistil except in the stigma. The strong preferential expression in vascular tissues suggests that AGP31 may be involved in vascular tissue function both during the defense response and development.




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