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Published on September 20, 2007; 10.1104/pp.107.103101

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Received June 3, 2007
Accepted September 11, 2007

Reduced Expression of Succinyl CoA Ligase can be Compensated for by an Upregulation of the {gamma}-amino-butyrate (GABA) Shunt in Illuminated Tomato Leaves

Claudia Studart-Guimarães , Aaron Fait , Adriano Nunes-Nesi , Fernando Carrari , Björn Usadel , and Alisdair R. Fernie *

Departments Willmitzer and Stitt, Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Golm, Germany

* Corresponding author; email: fernie{at}mpimp-golm.mpg.de.

Increasing experimental evidence suggests that the tricarboxylic acid cycle in plants is of greater importance in illuminated photosynthetic tissues than previously thought. In this study transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the {beta}-subunit of succinyl CoA ligase in either the antisense orientation or using the RNA interference approach, however, revealed little alteration in either photosynthesis or plant growth despite exhibiting dramatic reductions in activity. Moreover, the rate of respiration was only moderately affected in the transformants suggesting that this enzyme does not catalyse a crucial step in mitochondrial respiration. However metabolite and transcript profiling of these lines alongside enzyme and label redistribution experiments revealed that whilst considerable activity of this enzyme appears to be dispensable, the reason for such a mild phenotype in extremely inhibited lines was an upregulation of an alternative pathway for succinate production – that offered by the {gamma}-aminobutyric acid (GABA) shunt. When taken together these data highlight the importance both of succinate production for mitochondrial metabolism and the interplay between various routes of its production. The results are discussed in the context of current models of plant respiration in mitochondrial and cellular metabolism of the illuminated leaf.




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