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Published on July 13, 2007; 10.1104/pp.107.103143

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Received May 31, 2007
Accepted July 3, 2007

Characterization of AgMaT2, a Plasma Membrane Mannitol Transporter from Celery (Apium graveolens L.), Expressed in Phloem Cells, Including Phloem Parenchyma Cells

Marjorie Juchaux-Cachau , Lucie Landouar-Arsivaud , Jean-Philippe Pichaut , Claire Campion , Benoit Porcheron , Julien Jeauffre , Nathalie Noiraud-Romy , Philippe Simoneau , Laurence Maurousset , and Rémi Lemoine *

UMR CNRS 6161 < Transport des Assimilats >, Université de Poitiers, Bâtiment Botanique, 40 Avenue du Recteur Pineau, 86022 POITIERS CEDEX, France

* Corresponding author; email: Remi.Lemoine{at}univ-poitiers.fr.

A second mannitol transporter AgMaT2 was identified in celery (Apium graveolens L., var dulce), a species that synthesizes and transports mannitol. This transporter was successfully expressed in two different heterologous expression systems: baker yeast cells and tobacco plants (a non mannitol producing species). Data indicated that AgMaT2 works as an H+/mannitol cotransporter with a weak selectivity towards other polyol molecules. When expressed in tobacco, AgMaT2 decreased the sensitivity to the mannitol-secreting pathogenic fungi Alternaria longipes suggesting a role for polyol transporters in defence mechanisms. In celery, in situ hybridization showed that AgMaT2 was expressed in the phloem of leaflets, petioles from young and mature leaves, floral stem and roots. In the phloem of petioles and leaflets, AgMaT2, as localized with specific antibodies, was present in the plasma membrane of three ontologically related cell types: sieve elements, companion cells and phloem parenchyma cells. These new data are discussed in relation to the physiological role of AgMaT2 in regulating mannitol fluxes in celery petioles.




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E. J. Reidel, E. A. Rennie, V. Amiard, L. Cheng, and R. Turgeon
Phloem Loading Strategies in Three Plant Species That Transport Sugar Alcohols
Plant Physiology, March 1, 2009; 149(3): 1601 - 1608.
[Abstract] [Full Text] [PDF]


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