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Published on September 20, 2007; 10.1104/pp.107.103846

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Received June 12, 2007
Accepted September 7, 2007

Phage Type RNA Polymerase RPOTmp Transcribes the rrn Operon from the PC Promoter at Early Developmental Stages in Arabidopsis

Florence Courtois , Livia Merendino , Emilie Demarsy , Régis Mache , and Silva Lerbs-Mache *

Laboratoire Plastes et Differenciation Cellulaire, Université Joseph Fourier and Centre National de la Recherche Scientifique, B.P. 53, F-38041 Grenoble, France

* Corresponding author; email: Silva.Lerbs-Mache{at}ujf-grenoble.fr.

The plastid genome of higher plants is transcribed by two different types of RNA polymerases named NEP (Nucleus Encoded RNA Polymerase) and PEP (Plastid Encoded RNA Polymerase). PEP is a multimeric enzyme comparable to eubacterial RNA polymerases. NEP enzymes represent a small family of monomeric phage type RNA polymerases. Dicotyledonous plants harbour three different phage type enzymes, named RPOTm, RPOTp and RPOTmp. RPOTm is exclusively targeted to mitochondria, RPOTp is exclusively targeted to plastids and RPOTmp is targeted to plastids as well as to mitochondria. In the present paper, we have made use of RPOTp and RPOTmp T-DNA insertion mutants to answer the question of whether both plastid located phage-type RNA polymerases have overlapping or specific functions in plastid transcription. To this aim, we have analysed accD and rpoB mRNAs (transcribed from type I NEP promoters), clpP mRNA (transcribed from the -59 type II NEP promoter) and the 16S rRNA (transcribed from the exceptional PC NEP promoter) by primer extension. Results suggest that RPOTp represents the principal RNA polymerase for transcribing NEP controlled mRNA genes during early plant development while RPOTmp transcribes specifically the rrn operon from the PC promoter during seed imbibition.




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