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Published on July 6, 2007; 10.1104/pp.107.104000

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Received June 14, 2007
Accepted June 27, 2007

Mitochondrial Protein Lipoylation does not Exclusively Depend on the mtKAS Pathway of de-novo Fatty Acid Synthesis in Arabidopsis

Ralph Ewald , Üner Kolukisaoglu , Ursula Bauwe , Stefan Mikkat , and Hermann Bauwe *

Department of Plant Physiology, University of Rostock, Albert-Einstein-Straße 3, D-18059 Rostock, Germany; Core Facility Proteome Analytics, Medical Faculty, University of Rostock, Schilling-Allee 69, D-18057 Rostock, Germany

* Corresponding author; email: hermann.bauwe{at}uni-rostock.de.

The photorespiratory Arabidopsis mutant gld1 (now designated mtkas-1) is deficient in glycine decarboxylase (GDC) activity (Somerville and Ogren, 1982), but the exact nature of the genetic defect was not known. We have identified the mtkas-1 locus as gene At2g04540, which encodes ß-ketoacyl-[acyl carrier protein (ACP)] synthase (mtKAS), a key enzyme of the mitochondrial fatty acid synthetic system. One of its major products, octanoyl-ACP, is regarded as essential for the intra-mitochondrial lipoylation of several proteins including the H-protein subunit of GDC and the dihydrolipoamide acyltransferase (E2) subunits of two other essential multienzyme complexes, pyruvate dehydrogenase (PDH) and {alpha}-ketoglutarate dehydrogenase (KGDH). This view is in conflict with the fact that the mtkas-1 mutant and two allelic T-DNA knockout mutants grow well under non-photorespiratory conditions. Although on a very low level, the mutants show residual lipoylation of H-protein, indicating that the mutation does not lead to a full functional knockout of GDC. Lipoylation of the PDH and KGDH E2 subunits is distinctly less reduced than that of H-protein in leaves and remains unaffected from the mtKAS knockout in roots. These data suggest that mitochondrial protein lipoylation does not exclusively depend on the mtKAS pathway of lipoate biosynthesis in leaves and may occur independently of this pathway in roots.




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D. Feng, A. Witkowski, and S. Smith
Down-regulation of Mitochondrial Acyl Carrier Protein in Mammalian Cells Compromises Protein Lipoylation and Respiratory Complex I and Results in Cell Death
J. Biol. Chem., April 24, 2009; 284(17): 11436 - 11445.
[Abstract] [Full Text] [PDF]


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