Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on August 10, 2007; 10.1104/pp.107.104067

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
145/4/1171    most recent
pp.107.104067v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by De Buck, S.
Right arrow Articles by Depicker, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by De Buck, S.
Right arrow Articles by Depicker, A.
Agricola
Right arrow Articles by De Buck, S.
Right arrow Articles by Depicker, A.

Received June 19, 2007
Accepted July 18, 2007

Generation of Single-copy T-DNA Arabidopsis Transformants by the CRE/loxP Recombination-mediated Resolution System

Sylvie De Buck , Ingrid Peck , Chris De Wilde , Gordana Marjanac , Jonah Nolf , Annelies De Paepe , and Ann Depicker *

Department of Plant Systems Biology, Flanders Institute for Biotechnology, and Department of Molecular Genetics, Ghent University, 9052 Gent, Belgium

* Corresponding author; email: ann.depicker{at}psb.ugent.be.

We investigated whether complex T-DNA loci, often resulting in low transgene expression, can be resolved efficiently into single copies by CRE/loxP-mediated recombination. An SB-loxP T-DNA was constructed, containing two invertedly oriented loxP sequences located inside and immediately adjacent to the T-DNA border ends. Regardless of the orientation and number of SB-loxP-derived T-DNAs integrated at one locus, recombination between the outermost loxP sequences in direct orientation should resolve multiple copies into a single T-DNA copy. Seven transformants with a complex SB-loxP locus were crossed with a CRE-expressing plant. In three hybrids, the complex T-DNA locus was reduced efficiently to a single-copy locus. Upon segregation of the CRE recombinase gene, only the simplified T-DNA locus was found in the progeny, demonstrating DNA had been excised efficiently in the progenitor cells of the gametes. In the two transformants with an inverted T-DNA repeat, the T-DNA resolution was accompanied by at least a 10-fold enhanced transgene expression. Therefore, the resolution of complex loci to a single-copy T-DNA insert by the CRE/loxP recombination system can become a valuable method for the production of elite transgenic Arabidopsis thaliana plants that are less prone to gene silencing.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
T. Tzfira, S. V. Kozlovsky, and V. Citovsky
Advanced Expression Vector Systems: New Weapons for Plant Research and Biotechnology
Plant Physiology, December 1, 2007; 145(4): 1087 - 1089.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Verweire, K. Verleyen, S. De Buck, M. Claeys, and G. Angenon
Marker-Free Transgenic Plants through Genetically Programmed Auto-Excision
Plant Physiology, December 1, 2007; 145(4): 1220 - 1231.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists