Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on September 28, 2007; 10.1104/pp.107.104422

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
145/3/747    most recent
pp.107.104422v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alves-Ferreira, M.
Right arrow Articles by Meyerowitz, E. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alves-Ferreira, M.
Right arrow Articles by Meyerowitz, E. M.
Agricola
Right arrow Articles by Alves-Ferreira, M.
Right arrow Articles by Meyerowitz, E. M.

Received June 21, 2007
Accepted September 14, 2007

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

Marcio Alves-Ferreira , Frank Wellmer , Aline Banhara , Vijaya Kumar , Jose Luis Riechmann , and Elliot M. Meyerowitz *

California Institute of Technology, Division of Biology, Pasadena, CA 91125, USA; Department of Genetics, Federal University of Rio de Janeiro, Av. Prof. Rodolpho Paulo Rocco, CCS, 21949900 Rio de Janeiro, Brazil

* Corresponding author; email: meyerow{at}caltech.edu.

To obtain detailed information about gene expression during stamen development in Arabidopsis thaliana, we compared, by microarray analysis, the gene expression profile of wild-type inflorescences to those of the floral mutants apetala3, sporocyteless/nozzle, and male sterile 1, in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns.

Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild-type flowers of distinct developmental stages to those of the male sterile 1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage-specific repression of these genes.

Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4-kinase.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
X. Liu, J. Huang, S. Parameswaran, T. Ito, B. Seubert, M. Auer, A. Rymaszewski, G. Jia, H. A. Owen, and D. Zhao
The SPOROCYTELESS/NOZZLE Gene Is Involved in Controlling Stamen Identity in Arabidopsis
Plant Physiology, November 1, 2009; 151(3): 1401 - 1411.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Z. A. Wilson and D.-B. Zhang
From Arabidopsis to rice: pathways in pollen development
J. Exp. Bot., April 1, 2009; 60(5): 1479 - 1492.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Kurasawa, A. Matsui, R. Yokoyama, T. Kuriyama, T. Yoshizumi, M. Matsui, K. Suwabe, M. Watanabe, and K. Nishitani
The AtXTH28 Gene, a Xyloglucan Endotransglucosylase/Hydrolase, is Involved in Automatic Self-Pollination in Arabidopsis thaliana
Plant Cell Physiol., February 1, 2009; 50(2): 413 - 422.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M.-D. Huang, F.-J. Wei, C.-C. Wu, Y.-I. C. Hsing, and A. H.C. Huang
Analyses of Advanced Rice Anther Transcriptomes Reveal Global Tapetum Secretory Functions and Potential Proteins for Lipid Exine Formation
Plant Physiology, February 1, 2009; 149(2): 694 - 707.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Compiled by, F. Tooke, T. Chiurugwi, and N. Battey
Flowering Newsletter bibliography for 2007
J. Exp. Bot., July 18, 2008; (2008) ern109v1.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. D. Mara and V. F. Irish
Two GATA Transcription Factors Are Downstream Effectors of Floral Homeotic Gene Action in Arabidopsis
Plant Physiology, June 1, 2008; 147(2): 707 - 718.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists