Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on September 14, 2007; 10.1104/pp.107.104828

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
145/3/1073    most recent
pp.107.104828v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Welsch, R.
Right arrow Articles by Beyer, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Welsch, R.
Right arrow Articles by Beyer, P.
Agricola
Right arrow Articles by Welsch, R.
Right arrow Articles by Beyer, P.

Received June 28, 2007
Accepted September 10, 2007

The Transcription Factor RAP2.2 and its Interacting Partner SINAT2: Stable Elements in the Carotenogenesis of Arabidopsis Leaves

Ralf Welsch *, Dirk Maaß , Tanja Voegel , Dean DellaPenna , and Peter Beyer

Faculty of Biology, Center for Applied Biosciences, Universität Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA

* Corresponding author; email: welschra{at}web.de.

The promoter of phytoene synthase (PSY), the first specific enzyme of carotenoid biosynthesis, shows two main regulatory regions: a G-box containing region located near the TATA box, and a TATA box distal region containing the cis-acting element ATCTA which mediates strong basal promoter activity. This second element was also present in the promoter of phytoene desaturase (PDS), the next step of the carotenoid pathway, suggesting a common regulatory mechanism. In the present work, we demonstrate that AtRAP2.2, a member of the AP2/EREBP transcription factor family, binds to the ATCTA element. In Arabidopsis leaves, AtRAP2.2 transcript and protein levels were tightly controlled as indicated by unchanged transcript and protein levels in T-DNA insertion mutants in the AtRAP2.2 promoter and 5' UTR and the lack of change in AtRAP2.2 protein levels in lines strongly overexpressing the AtRAP2.2 transcript. Homozygous loss-of-function mutants could not be obtained for the AtRAP2.2 5' UTR T-DNA insertion line indicating a lethal phenotype. In AtRAP2.2 overexpression lines, modest changes in PSY and PDS transcripts were only observed in root-derived calli, which consequently showed a reduction in carotenoid content. The RING finger protein "SEVEN IN ABSENTIA OF ARABIDOPSIS 2" (SINAT2) was identified as an AtRAP2.2 interaction partner using a two-hybrid approach. The structure of SINAT2 and related proteins of Arabidopsis show homology to the "SEVEN IN ABSENTIA" protein of Drosophila that is involved in the proteasome-mediated regulation in a variety of developmental processes. The action of SINAT2 may explain the recalcitrance of AtRAP2.2 protein levels to change by altering AtRAP2.2 transcription.




This article has been cited by other articles:


Home page
 Plant CellHome page
C. I. Cazzonelli, A. J. Cuttriss, S. B. Cossetto, W. Pye, P. Crisp, J. Whelan, E. J. Finnegan, C. Turnbull, and B. J. Pogson
Regulation of Carotenoid Composition and Shoot Branching in Arabidopsis by a Chromatin Modifying Histone Methyltransferase, SDG8
PLANT CELL, January 1, 2009; 21(1): 39 - 53.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Bulow, S. Engelmann, M. Schindler, and R. Hehl
AthaMap, integrating transcriptional and post-transcriptional data
Nucleic Acids Res., January 1, 2009; 37(suppl_1): D983 - D986.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Den Herder, A. De Keyser, R. De Rycke, S. Rombauts, W. Van de Velde, M. R. Clemente, C. Verplancke, P. Mergaert, E. Kondorosi, M. Holsters, et al.
Seven in Absentia Proteins Affect Plant Growth and Nodulation in Medicago truncatula
Plant Physiology, September 1, 2008; 148(1): 369 - 382.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Newell-McGloughlin
Nutritionally Improved Agricultural Crops
Plant Physiology, July 1, 2008; 147(3): 939 - 953.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Welsch, F. Wust, C. Bar, S. Al-Babili, and P. Beyer
A Third Phytoene Synthase Is Devoted to Abiotic Stress-Induced Abscisic Acid Formation in Rice and Defines Functional Diversification of Phytoene Synthase Genes
Plant Physiology, May 1, 2008; 147(1): 367 - 380.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists