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Published on September 7, 2007; 10.1104/pp.107.105023

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Received July 2, 2007
Accepted September 4, 2007

Transcriptional Regulation of the Respiratory Genes in the Cyanobacterium Synechocystis sp. PCC 6803 during the Early Response to Glucose Feeding

Sanghyeob Lee , Jee-Youn Ryu , Soo Youn Kim , Jae-Heung Jeon , Ji Young Song , Hyung-Taeg Cho , Sang-Bong Choi , Doil Choi , Nicole Tandeau de Marsac , and Youn-Il Park *

Laboratory of Plant Genomics, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806; Department of Biology, Chungnam National University, Daejeon 305-764; Department of Biological Sciences, Myongji University, Yongin, Kyunggi-do 449-728; Department of Plant Science, Seoul National University, Seoul 151-742, Korea; Institut Pasteur, Unité des Cyanobactéries (CNRS URA 2172) 28, rue du Dr Roux, F-75724 PARIS cedex 15, France

* Corresponding author; email: yipark{at}cnu.ac.kr.

The coordinated expression of the genes involved in respiration in the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 during the early period of glucose treatment is poorly understood. When photoautotrophically grown cells were supplemented with 10 mM glucose in the light or after a dark adaptation period of 14 h, significant increases in the respiratory activity as determined by NAD(P)H turnover, respiratory O2 uptake rate, and cytosolic alkalization were observed. At the same time, the transcript levels of eighteen genes coding for enzymes associated with respiration increased with differential induction kinetics; these genes were classified into three groups based on their half-rising times. Transcript levels of the four genes gpi, zwf, pdhB, and atpB, started to increase along with a net increase in NAD(P)H, while the onset of net NAD(P)H consumption coincided with an increase in those of the genes tktA, ppc, pdhD, icd, ndhD2, ndbA, ctaD1, cydA and atpE. In contrast, the expression of the atpI/G/D/A/C genes coding for ATP synthase subunits was the slowest among respiratory genes and their expression started to accumulate only after the establishment of cytosolic alkalization. These differential effects of glucose on the transcript levels of respiratory genes were not observed by inactivation of the genes encoding the glucose transporter or glucokinase. In addition, several glucose analogues could not mimic the effects of glucose. Our findings suggest that genes encoding some enzymes involved in the central carbon metabolism and oxidative phosphorylation are coordinately regulated at the transcriptional level during the switch of nutritional mode.






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